中文摘要：

目的：研究肝癌高表达转录本（highly up-regulated in liver cancer,HULC）调控微小RNA613（micro RNA613,mi R-613）促进肝癌细胞增殖的作用机制。方法 ：①在Hep G2肝癌细胞株中,过量或抑制表达HULC,通过实时荧光定量聚合酶链反应（polymerase chain reaction,PCR）和荧光素酶报告基因系统检测mi R-613的表达量和活性,分析HULC与mi R-613之间的相互关系;②通过高通量表达芯片筛查mi R-613作用的靶基因,在Hep G2细胞株中应用化学合成的mi R-613模拟剂（mimic）和抑制剂（inhibitor）,用实时荧光定量PCR和蛋白印迹法分别检测靶基因和靶蛋白的表达情况;③HULC和mi R-613协同作用于肝癌细胞后,用流式细胞仪和软琼脂克隆形成试验观察肝癌细胞周期变化和克隆形成能力。结果：①在肝癌细胞中,HULC可调控mi R-613的表达,随着HULC表达量的增加,mi R-613表达和活性均降低;②Src基因是mi R-613作用的下游靶基因之一;③高表达HULC或抑制mi R-613表达可促进肝癌细胞周期改变和克隆形成。结论：肝癌细胞中HULC可调控mi R-613表达,通过Src基因相关的细胞信号转导途径,改变肝癌细胞的细胞周期等生物学特性。

英文摘要：

Objective： To study the mechanism of highly up-regulated in liver cancer（HULC） regulated micro RNA613 in promoting liver cancer cell proliferation. Methods： Expression and activity of micro RNA613 were detected by real-time PCR and luciferase assay when HULC was overexpressed or inhibited in liver cancer cell line Hep G2. The interaction between HULC and micro RNA613 was studied. The target gene of micro RNA613 was screened and confirmed with c DNA expression biochip. Furthermore, the biological characteristics affected by HULC/micro RNA613 in liver cancer cells such as cell cycle and colony forming ability were detected by flow cytometry and soft agar colony formation assay, respectively.Results： HULC could regulate the expression and activity of micro RNA613 in liver cancer cells. With the increased expression of HULC, micro RNA613 expression and activity were decreased. Src gene was one of the target genes of micro RNA613. Overexpression of HULC and inhibited expression of micro RNA613 could shorten cell cycle and affected the colony-forming ability in liver cancer cells. Conclusions： HULC could regulate the expression of micro RNA613 and change the cell cycle and proliferation of liver cancer cells via Src gene related cellular signal transduction pathway.