中文摘要：

糖基化是植物次生代谢产物生物合成中重要的修饰反应。目前已报道的以二萜为底物的UDP-糖基转移酶（UGT）数量稀少。本研究基于甜叶悬钩子叶片的转录组数据,利用生物信息学分析手段,选定可能具有二萜类催化活性的UDP-糖基转移酶基因进行克隆。最终克隆得到18条UGT基因序列,在大肠杆菌中进行了异源表达,并对克隆的基因进行了初步的序列分析。本研究为进一步挖掘和验证甜叶悬钩子中UGT的功能奠定了基础。

英文摘要：

Glycosylation is an important modification reaction in the biosynthesis of plant secondary metabolites. UDPglycosyltransferases（ UGT） consuming diterpenoid as substrates were scarcely reported. Based on transcriptic data of Rubus suavissimus S. Lee leaves,UGTs genes with potential diterpene catalytic activity was selected by bioinformatic analyses. Finally,18 UGT genes were successfully cloned and expressed in Escherichia coli. Preliminary sequence analysis of the cloned genes was performed. This study laid a foundation for further research and verification of the function of UGTs in Rubus suavissimus S. Lee.