通过盆栽试验,研究了鼠李糖脂和EDDS对黑麦草生长与吸收土壤重金属Cu、Zn、Pb和Cd,以及对土壤酶活性的影响。结果显示,向重金属复合污染土壤中施加1g·kg^-1的鼠李糖脂将显著降低黑麦草地上部的生物量。EDDS比鼠李糖脂具有更强的溶解土壤Cu、Zn、Ph和Cd的能力;同时施加1g·kg^-1的鼠李糖脂和0.4g·kg^-1的EDDS大幅增加了土壤溶液中Cu、Zn、Pb和Cd的浓度,显著增加了黑麦草地上部植株中Cu、Zn、Pb和Cd的含量,促进了土壤脲酶和脱氢酶的活性。鼠李糖脂与EDDS易生物降解,环境风险小,用于黑麦草修复重金属复合污染具有很大的修复潜力。
Phytoremediation has shown promising results in reducing the environmental risk of heavy metal polluted soft. This study is to optimize the experimental conditions of phytoremediation of a heavy metal contaminated soil assisted by chelates of EDDS/rhamnolipid. A series of pot experiments were carried out, and EDDS and Rhamnolipid were applied to the combined polluted soil to evaluate their effects on the growth of ryegrass, uptake of Cu, Zn, Pb and Cd by ryegrass, and soil enzymatic activities. The results showed that rhamnolipid( 1g·kg^-1) significantly decreased the biomass of ryegrass. EDDS had stronger ability in dissolving heavy metals from the soil than rhamnolipid. And the Cu, Zn, Pb and Cd concentrations in soil solution and in the above-ground part of ryegrass increased in response to the simultaneous supply of 1g·kg^-1 rhamnolipid and 0.4 g·kg^-1 EDDS. Meanwhile, rhamnolipid and EDDS stimulated the activities of soil urease and dehydrogenase. Thus, rhamnolipid and EDDS had great potential in remediation of the combined polluted soil, due to their easy biodegradation and relatively low toxicity to the environment.