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Synergistic effects of cellobiose dehydrogenase and manganese-dependent peroxidases during lignin degradation
  • 时间:0
  • 分类:O629[理学—有机化学;理学—化学]
  • 作者机构:[1]Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China, [2]S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510641, Peoples R China
  • 相关基金:This work was supported by the National Natural Science Foundation of China (Grant Nos. 20077015 and 29906005) ;by the Special Foundation of the Ministry of Education of China (Grant No. 200023).
中文摘要:

The synergistic effects of cellobiose dehydro-genase (CDH) and manganese-dependent peroxidases (MnP) on the degradation of kraft pulp cellulolytic enzyme lignin (CEL) were investigated. Addition of CDH significantly increased the amount of water-soluble products reduced from CEL by MnP. CDH facilitated the reduction of the contents of methoxyl, carboxyl, phenolic hydroxyl and total hydroxyl groups of CEL by MnP. 1H-NMR analysis showed that addition of CDH also decreased further the amount of protons of CEL degraded by MnP. The results proved for the first time that CDH could promote degradation of lignin by MnP and suggest that CDH could not only promote degradation of cellulose but also is an important part of the lignin biodeg-radation system.

英文摘要:

The synergistic effects of cellobiose dehydrogenase (CDH) and manganese-dependent peroxidases (MnP) on the degradation of kraft pulp cellulolytic enzyme lignin (CEL) were investigated. Addition of CDH significantly increased the amount of water-soluble products reduced from CEL by MnP. CDH facilitated the reduction of the contents of methoxyl, carboxyl, phenolic hydroxyl and total hydroxyl groups of CEL by MnP.1H-NMR analysis showed that addition of CDH also decreased further the amount of protons of CEL degraded by MnP. The results proved for the first time that CDH could promote degradation of lignin by MnP and suggest that CDH could not only promote degradation of cellulose but also is an important part of the lignin biodegradation system.

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