中文摘要：

单分子宽场光学显微成像技术是单分子检测技术的一种,具有通量高、参数多样、可实时动态监测等优点。本文评述了单分子宽场光学显微成像的技术方法、标记探针、判定原则、检测参数及其在分析化学、生物物理学等领域的应用,指出单分子成像技术正在向仪器设备的实用化、简易化,测量参数的精确化、可视化,研究范围的广泛化、复杂化等方面发展。未来几年单分子成像的研究重点可能会集中在实用定量、突破衍射极限的距离测量、重要生物过程的机理探索和纳米目标物的表征等方面。

英文摘要：

Among the techniques used for single molecule detection, wide-field optical microscopy offers the advantages of high throughput, multiple parameters and dynamic real-time monitoring. In the review, the wide-field optical microscopy apparatus, methods, detectable parameters, imaging probes, identifications criterion of single molecules, and applications in the fields of analytical and biophysics are summarized. Single molecule imaging is trending toward apparatus commercialization, operation simplicity, and process visualization with more accuracy in a wider and more complicated field. In the near future, the topics of single molecule detection will focus on quantitative analysis of biological sample, sub-diffraction-limit resolution, important biological mechanism, and characterization of nano-materials.