中文摘要：

目的：观察补阳还五汤对大鼠大脑中动脉阻塞（middlecerebralarteryocclusion，MCAO）后神经干细胞（neuralstemcells，NSCs）迁移的影响。方法：采用MCAO模型，缺血90min后再灌。将造模成功的大鼠分层并随机分组分为模型和补阳还五汤组，24h后ig补阳还五汤12g·kg-1，1次／d，连续3周。另16只不阻塞动脉大鼠设为手术组。分别在给药后1，2，3周进行行为学评分；并在第4天和18天ip5-溴-2-脱氧尿苷（5-bromo-2-deoxyuridine，BrdU）50mg·kg-1，各连续3d。2，3，5-三苯基四氮唑（TTC）染色测量梗死面积；免疫组化检测BrdU阳性细胞；酶联免疫法（ELISA）检测基质细胞衍生因子1（stromalcellderivedfactor-1，SDF-1）蛋白表达水平。结果：与假手术组比较，模型组1周和3周神经行为学评分显著升高（P〈0．05或P〈0．01），脑梗死面积、缺血侧室管膜下区（subventricularzone，SVZ）及纹状体区BrdU阳性细胞数均显著增加（P〈0．05或P〈0．01）。与模型组相比，补阳还五汤能显著改善大鼠MCAO神经功能缺失症状，药后1，2，3周神经功能评分为（1．33±0．60），（0．89±0．58），（0．50±0．31）分，明显低于模型组的（1．72±0．46），（1．56±0．51），（1．89±0．50）分（P〈0．01）；降低脑梗死面积，药后1，3周分别为（16．1±5．2）％，（10．3±0．40）％，明显低于模型组的（35．2±6．3）％，（29．8±6．9）％，（P〈0．01），提高SVZ和纹状体区BrdU阳性细胞数，药后1周分别为（71．17±5．19），（83．8±3．83）个，明显高于模型组的（52．17±6．52），（60．20±6．72）个，药后3周分别为（43．33±6．06），（54．60±5．77）个，明显高于模型组的（35.83±4．88），（22.00±3．87）个（P〈0．01）；给药3周时显著提高SDF-1表达，3周时为（36．3±2．71）pg·mg-1，明显高于模型组的（28．65±3．47）Pg·mg-1（P〈0．05

英文摘要：

Objective： To observe the effect of Buyang Huanwu decoction （BYHWD） on migration of neural stem cells after middle cerebral artery occlusion （MCAO） in rats. Method： Cerebral ischemia/reperfusion model of MCAO was established in rats using the suture method, successful modelings were divided into ischemia model group and BYHWD group randomly. BYHWD （ 12 g· kg-1 ） was administered orally 24 h after ischemia once a day for 21 days. Neurological deficit was assessed by neuroethological assessment. The area of cerebral was determined by 2, 3, 5-triphenyltetrazolium chloride （TTC） staining. 5-bromo-2-deoxyuridine （BrdU） positivecells were examined by immunohistochemistry, the expression of stromal cell derived factor-1 （SDF-1） measured by ELISA. Result： After MCAO the neurological behavioral score, cerebral ischemia area, the number of BrdU- positive cells Were significantly increased （P 〈 O. 05 or P 〈 O. O1 ）. Compared to model group, BYHWD could significantly reduce neurological behavioral score and cerebral ischemia areas （P 〈 O. 05 or P 〈 0. O1 ）, enhance the SDF-1 protein level （P 〈 0. 05）. The number of BrdU-positive cells in subventricular zone （SVZ） and corpus striatum also increased markedly （P 〈0. 05 or P 〈0. 01）. Conclusion： BYHWD improves migration of neural stem cells in ischemic penumbra after MCAO, the possible mechanism is related to the increased expression of SDF-1.