欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
利用自制的凹形电极在铂基底电极上直接构建了葡萄糖氧化酶微米点．首先，将电聚合和电化学刻蚀法相结合制备了凹形铂微米电极．然后将此种电极作为参比及辅助电极，基底铂电极作为工作电极，利用葡萄糖氧化酶在合适的条件下（浓度、一定量Triton X-100存在、电极电位等）由于电极表面pH的降低可以在铂电极上电沉积这一特性，将酶固定在铂基底电极上，微修饰得到了具有活性的葡萄糖氧化酶微米点．最终用扫描电子显微镜和扫描电化学显微镜对所得微米点进行了表征．所得微米点直径约20μm，且具有催化活性．该方法简便，干扰因素较少．
英文摘要：
Microscopic spots of glucose oxidase （GOx） were fabricated on platinum substrate electrode with a self-made concave platinum micrometer electrode. Firstly, the concave platinum micrometer electrodes were prepared by means of phenol-allylphenol copolymer insulation and electrochemical etching. Using concave platinum micrometer electrode as reference and counter electrodes and platinum substrate as a working electrode, GOx on the concave platinum electrode was then electrochemically deposited and fixed on a platinum substrate to form microscopic spots with biological activity by micro-decoration, based on the fact that GOx could be electrochemically deposited on the platinum electrode in a suitable condition （concentrations of GOx and Triton X-100, potential of electrode, etc.） due to the reduction of pH on the electrode surface. The GOx microscopic spots were characterized with scanning electron microscope and scanning electrochemical microscopy finally. The diameter of the microscopic spots fabricated by this method was about 20 μm, and the microscopic spots retained the biological activity. This approach of fabrication of GOx microscopic spots was simpler and had little interference.
同期刊论文项目
同项目期刊论文
期刊信息
位置：