中文摘要：

背景与目的前期体内实验证实肺岩宁方具有上调上皮细胞标志因子、Alpha-catenin、beta-catenin、E-Cadherin和下调间质细胞标志因子N-cadherin、Fibronectin的作用,而对Vimentin无明显作用。在此基础上,本研究旨在进一步以人高转移95-D肺癌细胞为研究对象,探讨肺岩宁方对上皮-间质细胞标志因子基因和蛋白表达的影响。方法采用不同浓度肺岩宁血清干预治疗人高转移肺癌细胞95-D的基础上,采用Real-timePCR及Westernblot方法检测对上皮-间质细胞标志因子Alpha-catenin、beta-catenin、E-Cadherin、N-cadherin、Fibronectin、Vimentin基因和蛋白表达的影响。结果 Real-timePCR结果表明：与对照组相比较,20%肺岩宁处理组Alpha-catenin表达上调（P〈0.05）,20%、25%肺岩宁处理组E-Cadherin表达上调（P〈0.05,P〈0.01）;各肺岩宁处理组beta-catenin表达无明显改变;5%、10%肺岩宁处理组间质细胞标志因子Vimentin表达下调（P〈0.01）,各肺岩宁处理组N-cadherin、Fibronectin无明显改变;Westernblot结果表明：5%、10%、15%、20%、25%肺岩宁处理组E-Cadherin蛋白表达上调（P〈0.01）,各肺岩宁处理组Alpha-catenin、beta-catenin蛋白表达无明显改变;5%、10%肺岩宁处理组N-cadherin、Fibronectin蛋白表达下调（P〈0.01）,各肺岩宁处理组Vimentin蛋白表达无明显改变。结论肺岩宁方通过调控部分上皮-间质细胞标志因子的表达,从而抑制肿瘤异质粘附能力和运动能力来参与肺癌的侵袭和转移。

英文摘要：

Background and objective Our previous in vivo experiment （paper has been published） has confirmed Feiyanning’s regulation effect on epithelial cell markers Alpha-catenin, beta-catenin, E-Cadherin and mesenchymal cell markers N-cadherin, Fibronectin, vimentin. Based on previous study, the objective of this study is to further investigate the expression of epithelial-mesenchymal cell marker gene and protein intervened by Feiyanning in highly metastatic lung cancer cells 95-D in vi-tro. Methods Human highly metastatic lung cancer 95-D cells were treated with different concentrations of Feiyanning in vitro, and then Real-time PCR and Western blot methods were used to detect mRNA and protein expression of epithelial-mesenchy-mal cell marker factor Alpha-catenin, beta-catenin, E-Cadherin, N-cadherin, Fibronectin and vimentin. Results Real-time PCR results showed that compared with the control group, Feiyanning at 20% concentration remarkably up-regulated the expression of Alpha-catenin （P〈0.05）, Feiyanning at 20% and 25% concentrations remarkably up-regulated the expression of E-Cadherin （P〈0.05, P〈0.01）, Feiyanning had no significantly effect on beta-catenin at any concentration, 5% and 10% Feiyanning at 5% and 10% concentrations significantly dow-regulated the expression of mesenchymal cell marker vimentin factor （P〈0.01）, Feiyan-ning had no regulatory role on N-cadherin and Fibronectin at any concentration. Western blot results showed that Feiyanning at 5%, 10%, 15%, 20% and 25% concentrations significantly increased E-Cadherin protein expression （P〈0.01）, but had no regulation effect on Alpha-catenin and beta-catenin protein expression; Feiyanning at 5% and 10% concentrations had a down-regulation effect on N-cadherin and Fibronectin protein expression （P〈0.01）, and had no regulation effect on vimentin proteinexpression. Conclusion Feiyanning play a role in inhibiting the adhesion of tumor heterogeneity and the athletic ability by regulating epithelial-mesenchymal cell marker f