中文摘要：

A sensitive and selective method based on gas chromatography hyphenated to mass spectrometry(GCMS) was developed and validated for the determination of atractylon in rat plasma.Plasma samples were processed by liquid-liquid extraction with ethyl acetate-n-hexane(1:1,v/v) using acetophenone as an internal standard(IS).Analytes were determined in selective ion monitoring(SIM) mode using target ions at m/z 108.1 for atractylon and m/z 105.1 for acetophenone.The calibration curve was linear over the concentration range of 10-1000 ng/mL with lower limit of quantification of 10 ng/mL The intra- and inter-day precision variations were not more than 10.4%and 9.6%,respectively,whilst accuracy values ranged from-6.5%to 4.9%.Extraction recovery of the assay was satisfactory.This method was successfully applied to quantification and pharmacokinetic study of atractylon in rat plasma after intragastric administration of Atractylodis extract.

英文摘要：

A sensitive and selective method based on gas chromatography hyphenated to mass spectrometry （GC- MS） was developed and validated for the determination of atractylon in rat plasma. Plasma samples were processed by liquid-liquid extraction with ethyl acetate-n-hexane （1：1, v/v） using acetophenone as an internal standard （IS）. Analytes were determined in selective ion monitoring （SIM） mode using target ions at m/z 108.1 for atractylon and m/z 105.1 for acetophenone. The calibration curve was linear over the concentration range of 10-1000 ng/mL with lower limit of quantification of 10 ng/mL. The intra- and inter-day precision variations were not more than 10.4% and 9.6%, respectively, whilst accuracy values ranged from -6.5% to 4.9%. Extraction recovery of the assay was satisfactory. This method was suc- cessfully applied to quantification and pharmacokinetic study of atractylon in rat plasma after in- tragastric administration of Atractvlodis extract.