中文摘要：

以花生品种花育20成熟胚萌发5～7d的幼叶为外植体，利用农杆菌介导法进行几丁质酶基因（CH5B）的遗传转化，并对转化条件进行了研究。结果表明，外植体经预培养2d，于OD600=0．6～0．8的农杆菌（GV3101）菌液中浸泡15min，再共培养3d，最适宜遗传转化。对抗性植株进行PCR检测，扩增得到了目的条带，表明几丁质酶基因成功转入了花生。

英文摘要：

CH5B gene was transferred into peanut cultivar Huayu No. 20 mediated by Agrobacterium tumefaciens , and transformation conditions were studied in this paper. The result showed that pre - culture for 2 days, Agrobacterium suspension （OD600 =0.6-0.8） for 15 min and co-culture for 3 days was suitable to obtain resistant shoots. The target band of resistant plants was amplified by PCR analysis. These results confirmed that CH5B gene was transferred into peanut successfully.