中文摘要：

目的：探讨外源性人低氧诱导因子（HIF）-1α基因在成纤维细胞中的表达及对体外培养人毛囊的影响。方法：通过脂质体将含有HIF—1α cDNA的真核表达载体pcDNA3．0瞬时转染成纤维细胞，应用反转录（RT）-PCR、免疫组化等方法检测HIF-1α在成纤维细胞中的表达。同时通过ELISA检测转染细胞上清液中血管内皮生长因子（VEGF）的表达情况。进一步将该上清加至体外培养的人毛囊和细胞中，显微镜下测量毛囊的平均生长长度，并观察毛囊的形态学变化。并通过四甲基偶氮唑蓝（MTT）法检测加入转染上清的细胞活性。结果：成功地将HIF—1α真核表达载体HIF—1α pcDNA3．0瞬时转染了成纤维细胞，用RT-PCR、免疫组化染色可检测出细胞中HIF—1α的表达，MTT检测转染后细胞活性增强，并且该上清液可以检测出VEGF的表达。该上清可以明显促进体外培养的人毛囊生长．延缓其进入退行期。结论：应用脂质体能够成功地将外源性人HIF—1α基因转染成纤维细胞，并进行有效表达，其表达的HIF—1α可增强细胞活性，且可诱导转染细胞上清液中VEGF的表达，在体外具有促进人毛囊生长的生物学活性。

英文摘要：

Objective： To investigate the feasibility of transient transfection of human HIF-1α gene into fibroblasts and the effects of the transfected cell culture supernatant on human hair follicle in vitro. Methods： HIF-1α pcDNA3.0 was transiently transfected into fibroblasts with lipofectamine^TM 2000. Expression of HIF-1α was observed by RT-PCR and immunohistochemistry. The expression of VEGF in the supernatant of transfected cells was detected by ELISA. Further, the supernatant was added to the cultures of human hair follicles and cells in vitro. MTF method was used to detect the cell viability. Results： HIF-1α pcDNA3.0 was successfully transfected into these fibroblasts. The expression of VEGF in the culture supernatant of cells transfected with HIF-1α was detected. Moreover, the specific culture supernatant of transfected fibroblasts accelerated the growth of human hair follicles and prolonged their anagen phase,and the activity of cells was enhanced. ConcIusions： HIF-1α pcDNA3.0 is successfully transfected into the fibroblasts. The expression of HIF-1α enhances the cellular activity and induces the VEGF expression, and the expression of VEGF upregulates the growth of human hair follicle.