中文摘要：

目的研究跨膜型血管内皮生长因子受体1（Flt-1）在子痫前期患者胎楹组织中的表达变化及其意义。方法采用RT—PCR方法检测20例子痫前期患者（观察组）胎盘组织中Flt-1 mRNA的相对表达量[以Flt-1与β肌动蛋白（β—actin）电泳条带吸光度（A）值的比值表示]，并以20例正常孕妇（对照组）为对照；采用蛋白印迹（western blot）法检测和比较两组各18例胎盘组织中Flt-1的蛋白相对表达量[其余4例标本因蛋白提取不理想而废弃，以Flt-1与磷酸甘油醛脱氧酶（GAPDH）电泳条带，4值的比值表示]。结果两组孕妇胎盘组织中均有Flt-1的表达。观察组孕妇胎盘组织Flt-1 mRNA相对表达量为2．25±0．19，对照组为1．23±0．29，两组比较，差异有统计学意义（P〈0．05）；观察组孕妇胎盘组织Flt-1蛋白相对表达量为2．67±1．19，对照组为0．94±0．51，两组比较，差异也有统计学意义（P〈0．05）。结论Flt-1可能参与子痫前期的病理生理过程。

英文摘要：

Objective To investigate the expression of fins-like tyrosine kinase receptor 1 （ Flt-1 ） in placentas of pre-eclampsia. Methods The expression of Flt-1 mRNA in the placentas from 20 preeclampsia patients and 20 pregnant women with normal blood pressure was detected by semi-quantitative reverse transcription-polymerase chain reaction. The protein expression of Flt-1 was analyzed using western blot in 18 pre-eclampsia patients and 18 normotensive pregnant women. Results Placental Flt-1 mRNA level in pre-eclampsia was 2. 25 ± 0. 19 （ intensity ratios of Fh-1 mRNA to β-actin mRNA） , significantly higher than in normotensive pregnant women 1.23± 0. 29 （ P 〈 0. 05 ）. Western blot showed that Flt-1 protein level in pre-eclamptic placenta was 2. 67 ± 1. 19 [ western blot signal intensity ratios of Flt-1 to glyceraldehyde-3-phosphate dehydrogenase （GAPDH） ] , significantly higher than in pregnant women with normal blood pressure 0. 94 ± 0. 51 （ P 〈 0. 05 ）. Conclusion Increased Flt-1 expression in pre-eclamptic placenta may be involved in pathogenesis of pre-eclampsia.