中文摘要：

目的：研究运动训练对脑梗死大鼠侧脑室室管膜下区（SVZ）内源性神经干细胞迁移的影响,并观察基质细胞衍生因子-α（SDF1-α）/趋化因子（CXCR4）信号通路在其中的作用.方法：成年Wistar大鼠81只,采用线栓法制作大脑中动脉阻塞再灌注模型,随机分为运动训练组（E,n=18）、运动训练＋AMD3100组（EA,n=18）、对照组（C,n=18）、对照＋AMD31 00组（CA,n=18）和假手术组（S,n=9）.E和EA组从术后第3天起每天予以跑笼运动训练;EA和CA组于术后第2天起隔天注射AMD3100;C、CA和S组则置于普通笼内饲养,不予以任何针对性措施.所有大鼠在造模术后第7、14、21d进行神经功能评分（mNSS）,并采用免疫荧光法观察大鼠梗死侧SVZ、纹状体、梗死周围皮质BrdU和Dcx双阳性细胞数,以及梗死边缘区SDF-1α及其受体CX-CR4的表达情况.结果：在造模术后第14天和第21天,E组的mNSS评分均明显优于其他各组（P＜0.05）.免疫荧光结果显示：术后第7、14、21天,E组BrdU和Dcx双阳性细胞及SDF-1α、CXCR4阳性细胞在相应区域的表达均多于C组（P＜0.05）、CA组（P＜0.001）和S组（P＜0.001）.此外,术后第14天和第21天,E组的BrdU和Dcx双阳性细胞数在各个区域的表达也明显多于EA组（P＜0.05）,E和EA组的SDF-1α、CXCR4阳性细胞在梗死边缘区的表达差异无显著性意义（P＞0.05）.结论：运动训练促进脑梗死大鼠神经功能的恢复,其机制可能与运动训练上调SDF-1 α/CXCR4的表达,进而促进SVZ的内源性神经干细胞迁移有关.

英文摘要：

Objective： To examine whether physical exercise can improve functional recovery by enhancing neural stem cells （NSCs） migration from subventricular zone （SVZ） through stromal-derived factor- 1 a （ SDF- 1 ct ）/chemokine re- ceptor-4（CXCR4） pathways in rats after ischemic stroke. Method： Eighty-one adult Wistar rats after transient middle cerebral artery occlusion（tMCAO） were randomly divided into five groups： physical exercise group （E, n=18）, physical exercise combined with AMD3100 injec- tion group （EA, n=18）, Both of these two groups were given running exercise daily at 3 days after MCAO. Control group （C, n=18）, control combined with AMD3100 injection group （CA, n=18） and sham-operated group （S, n=9）, which all were fed in standard cages without any special training exercise. The rats were sacri- ficed on the 7th, 14th, 21st day after evaluating ~the neurological function with modified neurological severity score（mNSS）. Bromodeoxyaridine（BrdU） and doublecirtin（Dcx） double-positive cells were observed in the ipsi- lateral SVZ, striatum and peri-infarct cortex. SDF-lct and CXCR4 were checked in the peri-infarct region. Result： The mNSS score in E group was much better than that in other groups （P〈0.05） at the 14th days and 21st days after MCAO （P〈0.05）. Immunofluorescence analysis showed amount of BrdU and Dcx double posi- tive ceils and SDF-lct, CXCR4 positive cells displayed a significantly higher expressions in E group than that in C group （P〈0.05）, CA group（P〈0.001） and S group （P〈0.001） at each time points and that in EA group at the 14th and 21st days （P〈0.05） after MCAO in the corresponding regions. In addition, physical exercise signif- icantly increased the amount of SDF-lct and CXCR4-positive cells but no significant difference（P〉0.05）. Condusion： Physical exercise can enhances neurological function possibly by mediating endogenous NSCs mi- gration from SVZ to the peri-infarct region via SDF-la/CXCR4