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中文摘要：
为了探究高致病性猪蓝耳病病毒（HP-PRRSV）的遗传变异特征，本研究对GenBank中235株HP—PRRSV GP5序列的遗传进化、主要氨基酸基序、抗原性以及N-糖基化位点数量和位置的变异进行了分析。结果表明HP-PRRSV之间的同源性较高，与其他参考毒株相比，这些病毒处于一个相对独立的分支中，而且与经典疫苗株的亲缘关系较远。这有助于解释经典疫苗株对于HP—PRRSV为何起不到理想的免疫保护效果。在病毒的中和表位序列中存在着规律的点突变，同时抗原性比较显示HP-PRRSV与经典毒株之间存在着一定的差异。绝大多数的HP-PRRSV的N-糖基化位点在数量上多一个，而且位置要向羧基端平移2个氨基酸，从而使得中和表位两侧直接与糖链相连，可能会造成中和表位被糖侧链所遮掩，本研究由此推测减弱中和抗体对HP-PRRSV的有效识别，促进病毒逃避机体体液免疫。
英文摘要：
A highly pathogenic porcine reproductive and respiratory syndrome （HP-PRRSV） was emerged in China since the summer of 2006. In order to investigate the genetic diversity of HP-PRRSV, we analyzed 235 GP5 sequences of HP-PRRSVs deposited in GenBank and compared with those of homologous amino acid neutralizing among themselves, but genetically distinct classical from the strains. The results showed that the HP-PRRSVs were highly vaccine strains which were derivated from classical mutation occurred in the prime neutralizing epitope and an additional N-glyscan chain was found close epitope. It was proposed that this N-glyscan chain could contribute to the viral immunological escape PRRSVs. An to the prime from humoral immunity.
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