位置:成果数据库 > 期刊 > 期刊详情页
D101型大孔吸附树脂纯化油茶皂苷的工艺研究
  • 期刊名称:中国药房. 2009, 20(12): 900-902.
  • 时间:0
  • 分类:R284.2[医药卫生—中药学;医药卫生—中医学] R943[医药卫生—药剂学;医药卫生—药学]
  • 作者机构:[1]大连大学生物工程学院,大连市116622
  • 相关基金:国家自然科学基金青年科学基金资助项目(30801509)
  • 相关项目:茶麸皂素抗肿瘤有效成分及作用机理研究
中文摘要:

目的:研究D101型大孔吸附树脂纯化油茶粕中油茶皂苷的工艺。方法:以油茶皂苷的含量为指标,考察D101型大孔吸附树脂的上样量、吸附容量和水溶性杂质、油茶皂苷洗脱的乙醇浓度及体积。结果:纯化工艺:D101型大孔吸附树脂的上样量为3BV,吸附容量为566mg·g^-1生药量,使用3BV蒸馏水和1BV20%乙醇洗脱水溶性杂质,3BV85%乙醇洗脱油茶皂苷。结论:D101型大孔吸附树脂可较好地纯化油荼皂苷,该工艺简单,成本低,适用于工业化大生产.

英文摘要:

OBJECTIVE: To study the purification technology of camellia saponin using D101 macroporous adsorption resin. METHODS: The loading volume of sample and the adsorption quantity of D101 macroporous adsorption resin and the concentration and volume of alcohol in eluting water soluble foreign substances and total cemallia saponins were investigated taking the content of total cemallia saponins as index. RESULTS: The loading volume of sample of D101 macroporous adsorption resin was 3 fold that of resin D101 (or 3 BV, resin bed volume) and its adsorption quantity was 566 mg · g^-1(crude drug) ;3 BV distilled water and 1 BV 20% ethanol were used for the elution of the water-soluble foreign substances, and 3BV of 85% ethanol was used for the elution of total cemallia saponins. CONCLUSION: D101 macroporous resin has good purification efficacy for camellia saponins. The purification process is simple with low cost, and it is applicable for large - scale industrialized production.

同期刊论文项目
同项目期刊论文