中文摘要：

目的探讨槲皮素对凝血酶诱导大鼠血管平滑肌细胞（VSMC）增殖和迁移的影响及其机制。方法取第3～5代体外培养的大鼠胸主动脉VSMC，分为对照组（在DMEM培养基中加入磷酸盐缓冲液）、凝血酶组（在DMEM培养基中加入1 U/ml凝血酶）和槲皮素组（在DMEM培养基中先加入1 U/ml凝血酶刺激后，再加入100 μmol/L槲皮素）。采用CCK－8法检测细胞的增殖能力；Transwell小室法检测细胞的迁移能力；Western blot法检测磷酸化细胞外信号调节激酶1/2（ERK 1/2）和磷酸化p38蛋白的表达水平。结果（1）CCK－8法检测显示，凝血酶组的吸光度值高于对照组（2.59±0.16比1.97±0.18，P〈0.01）；槲皮素组的吸光度值（2.13±0.19）低于凝血酶组（P〈0.01）；槲皮素组与对照组的吸光度值差异无统计学意义（P〉0.05）。（2）Transwell小室法检测显示，凝血酶组VSMC的迁移细胞数多于对照组[（1 337±162）个比（99±26）个，P〈0.01]；槲皮素组VSMC的迁移细胞数为（926±111）个，少于凝血酶组（P〈0.05）；槲皮素组VSMC的迁移细胞数多于对照组（P〈0.01）。（3）凝血酶组的磷酸化ERK 1/2和磷酸化p38蛋白表达水平均高于对照组（P均〈0.05）；槲皮素组的磷酸化ERK 1/2和磷酸化p38蛋白表达水平均低于凝血酶组（P均〈0.05）；槲皮素组与对照组的磷酸化ERK 1/2和磷酸化p38蛋白表达水平差异无统计学意义（P均〉0.05）。结论槲皮素可抑制凝血酶诱导的大鼠VSMC增殖和迁移，其机制可能与抑制ERK 1/2和p38磷酸化有关。

英文摘要：

Objective To investigate the effects and related mechanism of quercetin on thrombin- induced proliferation and migration of rat vascular smooth muscle cells （VSMCs）. Methods Third to fifth generation VSMCs were divided into three groups, including control group （ with PBS on the base of medium DMEM）, thrombin group （with 1 U/ml thrombin on the base of medium DMEM） and quercetin group （treated with 100 μmol/L quercetin before 1 U/ml thrombin on the base of medium DMEM）. The proliferation and migration eapacitities were tested by CCK-8 kit and transwell chamber, respectively. The protein level of phospho-extraeellular signal regulated kinase 1/2 and phosphor-p38 were measured by Western blot. Results （ 1 ） According to the CCK-8 results, optical density value was significantly higher in thrombin group than that of control group （ 2. 59± 0. 16 vs. 1.97 ± 0. 18, P 〈 0. 01 ）, which could be significantly attenuated by pretreatment with quercetin （2. 13 ± 0. 19, P 〈 0. 01 ）, and there was no significant difference between quereetin group and control group（ P 〉 0. 05 ）. （2） The transwell results showed that the migrated VSMCs were significantly higher in thrombin group than in control group （ 1 337 ± 162 vs. 99 ±26, P 〈 0.01 ） , which could be significantly reduced by pretreatment with quercetin （ 926 ± 111, P 〈 0.05 ） , but still significantly higher than control group （ P 〈 0. 01 ）. （ 3 ） The protein expressions of phospho- extraeellular signal regulated kinase 1/2 and phosphor-p38 were significantly upregulated in thrombin groupcompared to control group （ both P 〈 O. 05 ）, which could be significantly downregulated by pretreatment with quercetin （P 〈 O. 05）. Conclusion Quercetin can effectively attenuate thrombin-induced vascular smooth muscle cells proliferation and migration, possibly through inhibiting the phosphorylation of extracellular signal regulated kinase 1/2 and p38 pathway.