中文摘要：

目的探讨补阳还五汤对大鼠脑内移植神经干细胞（NSCs）治疗局灶性脑缺血的影响。方法分离培养新生大鼠海马NSCs，用5-溴-2-脱氧尿嘧啶核苷（BrdU）进行标记。采用大脑中动脉线栓法复制脑缺血模型；将实验大鼠随机分为模型组、空白移植组、单纯移植组和移植加中药组，移植组大鼠术后3d向脑室注射10μl NSCs细胞悬液，空白移植组则注射等量生理盐水。于移植前及移植后7、14、28d观察神经功能评分，相应时间点处死大鼠后用免疫组化法检测移植细胞存活情况，用荧光双标记免疫组化法检测BrdU和神经丝蛋白200（NF200）双阳性细胞数。结果单纯移植组大鼠神经功能明显得到恢复，在14d和28d与模型组比较差异有统计学意义（P均〈0．05）；移植加中药组则提前到7d（P均〈0．05）；模型组与空白移植组比较神经功能评分差异无统计学意义。镜下观察NSCs能移行至缺血部位并存活，移植加中药组存活和新生细胞在14d和28d与单纯移植组比较差异有统计学意义（P〈0．05或P〈0．01）。结论联合使用补阳还五汤能促进脑缺血后移植NSCs的存活，提高新生神经元数量，促进神经功能的恢复。

英文摘要：

Objective To investigate the effects of Buyang Huanwu decoction （补阳还五汤） on transplantation of neural stem ceils （NSCs） into brain for treatment of focal cerebral ischemia in rats. Methods Hippocampus NSCs were isolated and cultured in vitro, then labeled with 5-bromo-2-deoxyuridine （BrdU）. The model of brain ischemia was induced by middle cerebral artery occlusion. The animals were randomly divided into four groups： model group, sham transplantation group, simple transplantation group and transplantation with traditional Chinese medicine （TCM） group. Three clays after the middle cerebral artery occlusion, 10 μl suspension of BrdU-labeled NSCs was injected into a lateral ventricle in rats in the simple transplantation group, while a similar amount of normal saline was injected into the same brain location in rats in the sham transplantation group. At post-operative 7, 14 and 28 days, the rats were sacrificed respectively. The neurological deficit scores were determined, and the survival situation of the transplanted NSCs was detected by immunohistochemical staining. Results The simple transplantation of NSCs could improve the neurological behavior performance obviously, the differences being significant at 14 and 28 days between the simple transplantation group and model group and between the former group and sham transplantation group （all P〈0. 05）. Under microscope, the NSCs could be found to migrate into the ischemic zone and survive. The numbers of living cells and regenerating cells in Buyang Huanwu decoction （TCM） ＋ transplantation group were significantly higher than those in simple transplantation group at 14 and 28 days （P〈0. 05 or P〈 0. 01 ）. Conclusion The transplantation of neural stem cells combined with Buyang Huanwu decoction can promote the survival of transplanted neural stem cells and increase the number of regenerating neurons in ischemia brain, thus, enhance the recovery of neurological function in rat models with focal cerebral ischemia.