将由焦化活性污泥中提取的1株吡啶降解菌BC026、1株喹啉降解菌BW004及2株咔唑降解菌BC039和BC046,按一定比例配制成复合菌剂M19,应用于吡啶、喹啉和咔唑的同步降解,探讨3种氮杂环芳烃化合物的降解机理。实验结果表明:M19的同步降解效率比单菌株降解效率高,其中喹啉降解中间产物与单菌降解结果相同;4株菌在复合条件下生长良好,且喹啉降解基因和咔唑降解基因得到保持。M19对3种污染物的同步降解过程是各株降解菌作用的加合,但由于消除了各污染物对非其降解菌的抑制作用,菌株之间表现为一种互利关系。
A microbial consortium M19,originated from four degrading bacteria for degrading pyridine(Shinellazoogloeoides BC026),quinoline(Pseudomonas sp.BW004),and carbazole(Pseudomonas sp.BC039 and BC046) were used to explore the simultaneous degradation mechanism of nitrogen heterocyclic compounds(NHCs).The experimental results showed that the simultaneous degradation efficiency of the three NHCs by M19 was higher than those by any single strain.The intermediate products from quinoline degraded by M19 were the same as those by BW004.All four bacterial strains grew well in the media containing three NHCs,and the quinoline degradation gene(qorL) and carbazole degradation gene(carAa) had been held and expressed.The mechanism of NHCs' simultaneous degradation by M19 was the simple superposition of the 3 degradation processes carried by each strain,however,the strains benefited from each other due to the rapid reduction of toxicity from NHCs.