采用盆栽砂培试验,研究了不同浓度氯离子(Cl-)处理对油菜(Brassica napus L.)幼苗干物质积累速率(DMAR)、根冠比(R/S)、叶绿素(Chl)含量、氯离子含量(Cl-)、净光合速率(Pn)、气孔导度(Gs)、细胞间CO2浓度(Ci)、蒸腾速率(Tr)、水分利用效率(WUE)、气孔限制值(Ls)等的影响。结果表明,25~100 mmol/L Cl-处理9 d和18 d,油菜幼苗植株DMAR均上升,均在50 mmol/L Cl-处理时达到最大值;不同浓度Cl-处理9 d,Chl含量有先上升后下降的趋势,在100 mmol/L Cl-处理时达到最大值,处理18 d,Chl含量依次下降。Cl-处理能显著提高叶片中的Cl-含量。除了在处理18 d后的Ci外,25~100 mmol/L Cl-处理下,油菜叶片均能维持较高的Pn、Gs、Ci和Tr,200 mmol/L Cl-处理9 d和18 d,油菜叶片Pn、Gs、Ci和Tr均显著下降。随着Cl-浓度的增加,WUE呈现先降后升,在9 d处理后,随着Cl-浓度的增加,油菜Ls也呈现先升后降,而在18 d处理后,随着Cl-浓度的增加,Ls逐渐显著上升。上述结果表明,外施低量氯(Cl-25~100 mmol/L)显著提高或者维持油菜幼苗的光合作用和蒸腾作用,促进油菜幼苗的生长;而高氯(Cl-200 mmol/L)条件下显著降低其光合作用和蒸腾作用,抑制其生长,且抑制油菜幼苗光合的主要是气孔因素。相关性分析还表明,植株DMAR、Pn、Ci、Tr、Gs之间均有显著的正相关性,而Pn与WUE之间存在极显著的负相关。
Soil salinization not only is a serious environmental problem but also poses a severe threat to plant growth and agricultural production.The ions of Na+ and Cl-are mainly responsible for salinization.People generally pay close attention to study the Na+ effects on grow,productivity and quality of crop plant,however,relatively much less studies are on Cl-effects on crop plant.The effects of different concentrations of Cl-(0,25,50,75,100 and 200 mmol/L) on plant growth,photosynthetic pigment,photosynthesis traits and water use efficiency in canola(Brassica napus L.) seedlings for 9 and 18 days were investigated.The results show that the dry matter accumulation rates are increased under the 25-100 mmol/L Cl-treatments for 9 and 18 d,and the highest values are under the treatment of 50 mmol/L Cl-,while the plant dry matter accumulation rate is decreased significantly under the 200 mmol/L Cl-.The chlorophyll(Chl) contents are ascended in first and descended under different concentrations of Cl-treatment for 9 d,and the highest values are under the treatments of 100 mmol/L Cl-.Nevertheless,for 18 d,the Chl contents are decreased under the Cl-treatments,and the more the applied Cl-concentration is,the more the decrease is.The chloride concentrations of canola leaves are markedly increased under the Cl-treatments.Compared with the control,the Cl-contents in canola leaves are increased by 814%,805%,1043%,1083% and 2157% for 9 d and by 745%,1270%,1340%,1900 and 3386% for 18 d,under 25,50,75,100 and 200 mmol/L Cl-treatments,respectively.Apart from Ci under the Cl-treatments for 18 d,canola leaves are able to maintain high values of Pn,Gs,Ci,and Tr under 25-100 mmol/L Cl-treatments.For 9 and 18 d,Pn both achieve the highest values under the 25 mmol/L Cl-treatment,and are increased by 12% and 13% compared with each control,respectively.Pn,Gs,Ci and Tr of canola leaves are significantly decreased under the 200 mmol/L Cl-treatment for 9 and 18 d.As Cl-concentration increased,WUE is descended in first and ascende