中文摘要：

以含柔嫩艾美耳球虫rhomboid基因的重组质粒为模板，应用PCR方法扩增该基因完整开放阅读框，克隆至pMD18-T载体中。分别用限制性内切酶PstⅠ／ClaⅠ和PvUⅡ／ClaⅠ进行双酶切，将rhomboid目的基因克隆到大肠杆菌-分支杆菌穿梭表达载体pMV261和整合表达载体pMV361中，获得重组质粒pRho-261和pRho-361。将重组质粒电穿孔转化卡介苗，经热诱导后对其表达产物进行SDS—PAGE和western blot分析。结果表明成功构建了两个重组质粒，其表达产物与柔嫩艾美耳球虫阳性血清具有免疫反应性，为重组卡介苗在鸡球虫病防治方面的研究奠定了基础。

英文摘要：

The open reading frame （ORF） of Eimeria tenella （E. tenella） rhomboid gene was amplified by PCR and cloned into the pMV261 and pMV361 expression vectors, respectively. The resulting plasmids, pRho-261 and pRho-361 were transformed into BCG by electroporation. The recombinant rhomboid protein was successfully expressed in rBCG pRho-261 and pRho-361 and can be recognized by E.tenella positive serum in western blot assay. This study established a foundation for further study on recombinant BCG vaccination against coccidiosis in chicken.