采用“三级十倍环式稀释法(three level ten-fold dilution method)”的方法,对取自添加了DDT、林丹的盆栽实验土壤中的原生动物丰度进行研究分析,以此作为典型POPs物质对土壤原生动物的抑制效应的初步探索性研究。实验发现,在添加了DDT、林丹的土壤中的原生动物的丰度与对照相比有大幅度的下降,其种群结构遭到了相当大的破坏。其中作为优势类群的鞭毛虫、肉足虫平均数量最小时仅为CK对照的0.3%、2.5%,而对污染最为敏感的纤毛虫则在土壤中完全消失。研究结果表明,受典型POPs物质污染的土壤,即使其含量很低,也对原生动物的抑制作用非常显著。
Soil protozoa play an important role in transformation in soils. However, the difficulty in terrestrial environments at present. In this regard soil ecosystem, especially making contributions to material cycling and energy counting and taxonomic analysis limits the use of soil protozoa as bioindicator in , there is little literature available on the effects of POPs on soil protozoa.In order to acquire primary understanding of the toxicity of typical POPs on soil protozoa, the effects of DDT and lindane on the community structure and abundance of soil protozoa were assessed. Pot experiments simulating farm ecosystem were begun with the uncontaminated soils in China Agricultural University, the pesticides were then mixed into the soil at concentrations of 1, 10, 50 mg· kg^-1 dry soil for lindane and 1, 10, 50 mg· kg^- 1 dry soil for DDT. Three replicates were used for each dose. The same procedure was employed to prepare a set of three control replicates without the pesticides. The pots were placed in a greenhouse for 60 days, and the abundance of soil protozoa was analyzed with the "three level ten-fold dilution method" at days 1, 3, 7, 15, 30 and 60, and the residue, level of lindane and DDT were analyzed. The results showed that the soil protozoa were sensitive to DDT and lindane. Compared with control group, the species and numbers of protozoa in soil samples treated by DDT and lindane decreased markedly, and the population structure and community were greatly demolished. The minimal abundance of flagellate and sarcodina in the DDT and lindane group was only 0.3 % and 2.5 % compared to that of control group, respectively, and the ciliates disappeared completely. The results suggested that the typical POPs in soil, even with low concentrations, could be greatly harmful to soil protozoa.