以滤纸染毒和溶液染毒法研究了喹乙醇对蚯蚓的急性毒性效应以及对其生长的影响。结果表明,滤纸染毒和溶液染毒48h的致死中浓度(LC50)分别为1.02mg·cm^-2和〉4000mg·L^-1喹乙醇能引起蚯蚓的形态病理变化。低浓度喹乙醇(500、1000mg·kg^-1)对蚯蚓的体重增长率没有显著影响(P〉0,05),高浓度喹乙醇(3000、5000mg·kg^-1)能极显著降低蚯蚓体重增长率(P〈0.01)。检测暴露喹乙醇14d的蚯蚓体和不同部位的过氧化氢酶(CAT)活性,结果发现,高浓度喹乙醇能显著抑制蚯蚓体及其前部和中部CAT的活性。低浓度喹乙醇只对蚯蚓前部的CAT活性表现出显著抑制(P〈0.05)。
By using standard earthworm acute toxicity test, including filter paper test and solution test, the toxicity of olaquindox to earthworm was assessed. The results showed that the 48 h median lethal concentration (LC50) was 1.02 mg·cm^-2 on filter paper and above 4 000 mg·cm^-1 in solution. The morphological changes of earthworm, especially anterior segment, showed a progression of damage with increasing concentrations of olaquindox in agents, which conformed to the result in solution test. In addition, compared to the control, weekly growth rate of earthworm revealed no significant effect (P〉0.05) under 7 days or 14 days exposure to lower concentrations (500 mg·kg^-1g, 1 000 mg·kg^-1), but reduced significantly under 7 days or 14 days exposure to higher concentrations(3 000 mg·kg^-1 and 5 000 mg'kg-')(P〈0.01). Catalase (CAT) activity in whole earthworm and its various segments were measured to determine further toxicity of olaquindox to earthworm after 14 days exposure. At two higher concentrations after 14 days exposure, CAT activity in the whole earthworm, the anterior and the mid-part was inhibited significantly, while, at two lower concentrations, CAT activity exhibited the significant reduce only in the anterior compared to the control (P〈0.05), suggesting CAT activity in the anterior was more sensitive to olaquindox than other segments. This may help us to explore biomarker for early warning signals of veterinary medicine on soil pollution.