中文摘要：

目的对免疫损伤介导的再生障碍性贫血（再障）小鼠模型进行改良研究，为其发病机制和治疗提供研究平台。方法Balb／c小鼠（受鼠）经5．0Gy ^60Co叫射线照射后，输入5×10^6个DBA／2小鼠（供鼠）的淋巴结细胞，观察外周血细胞、骨髓组织切片、骨髓单个核细胞计数、血清IFN-γ水平和调节T细胞（Treg）等指标。结果照射和淋巴结细胞输入可迅速导致Balb／c小鼠外周血三系严重减少，在输入供鼠淋巴结细胞后第14天最低，第28天仍呈三系严重减少，无恢复迹象。第14天骨髓组织切片出现骨髓增生极度减低、造血细胞罕见、脂肪细胞填充等表现，至第28天均未出现改善。血清IFN-γ浓度于第6天[（170．0±17．0）pg／ml]明显升高，为正常水平[（27．7±7．1）pg／m1]的6．3倍，随后逐渐下降至正常；Treg数量先降低，后逐渐上升，第21天[（3．38±0．52）％]基本恢复正常水平[（4．04±0．44）％]；在第14天检测Foxp3，与正常对照组比较差异无统计学意义（P〉0．05）。结论通过对受鼠进行放射性核素照射和供鼠淋巴结细胞输注，模型小鼠的各项检测指标均达到骨髓衰竭的诊断标准，符合骨髓衰竭的细胞免疫损伤的发病特点，可作为深入研究骨髓衰竭发病机制及治疗的小鼠模型。

英文摘要：

Objective To establish a mouse model for the study of pathophysiologic mechanism and treatment of borne marrow failure（BMF）. Methods Balb/c mice （recipient） were irradiated 5.0 Gy by γ rays of 60^Co, and then infused 5 × 10^6 lymph node（LN） cells from DBA/2 mice （donor） in 4 hours. Pancytopenia was monitored by cell counting, bone marrow damage was assessed by histological staining and mononuclear cell counting. Serum IFN-γ coneentration was measured by ELISA. The proportion of Treg in spleen was detected by flow cytometry. Results h＇radiation and infusion of LN cells led to rapid development of severe pancytopenia and BM hypoplasia, which reached the most severity at d14. The pancytopenia remained at d28 and displayed no signs of recovel7. The bone marrow was full of adipose cells with scarcity of hematopoi- etic ceils at d14 and persisted at least for 28 days, being similar to the feature of aplastic anemia. Serum IFN-γ＇ concentration was 6.3 fold increased [ （ 170.0 ± 17.0） vs （ 27.7 ± 7. 1 ） pg/ml ] at d6. Tregs were decreased after infusion, and then increased [ （3.38 ± 0.52）% ] and recovered to normal [ （4.04± 0.44）% ] at d21. The expression level of the specific transcription factor Foxp3 was similar to normal. Conclusion The MHC antigen of Balb/c mice is identical to that of DBA/2 mice, but their minor antigen differs. 5.0 Gy irradiation and then 5 ×10^6 lymphocyte infusion can induce BMF similar to the features of aplastic anemia.