中文摘要：

目的：研究隐球菌与体外培养的角质形成细胞的相互作用。方法：检测新生隐球菌对体外培养的角质形成细胞HaCaT株（简称HaCaT细胞）的时间-浓度黏附率、通透率；检测新生隐球菌对细胞的损伤；透射电镜观察二者相互作用的超微结构。结果：新生隐球菌标准野生株B3501（简称B3501）可以对HaCaT细胞产生黏附与侵袭，黏附率与侵袭率呈现时间依赖性；同时，B3501还可以使HacaT细胞凋亡率升高，对其造成损伤，这与菌体的活力相关。超微结构可见新生隐球菌与角质形成细胞的黏附与侵袭过程。结论：活的隐球菌黏附与侵袭角质形成细胞是其感染皮肤的重要条件。进一步明确二者的相互作用对隐球菌发病机制的研究具有重要意义。

英文摘要：

Objective： To investigate in vitro interaction between Cryptococcus neoforrrtans and keratinocytes. Methods： Cryptococcus neoforrrtans and keratinocytes were co-cultured in vitro and the ratio of Cryptococcus neoformans adherence to, invading of, and damage to keratinocytes were measured respectively. Transmission electron microscope was used to identify the uhrastructure of their interactions. Results： Cryptococcus neoformans （B3501） showed adherence to, invading of and damage to keratinocytes, which acted in a time-dependent manner, meanwhile B3501 could up-regulate apoptotic rates of keratinocytes which had relation with viability of Cryptococcus neoforrrtans. Ultrastructure of the interactions revealed Cryptococcus neoforrrtans could survive and bud in the cell. Conclusion： It is essential for the infection establishment of the skin that viable Cryptococcus neoforrrtans could adhere to, invade and damage keratinocytes, and further study should be done to illuminate the mechanism of cryptococcal skin infection.