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中文摘要：
整体柱是通过在柱管内原位聚合或固化的方法制备得到的具有多孔结构的整体棒状固定相，与传统的填充柱相比，具有通透性好、传质速率快、容易制备等优点，因此在分离分析领域特别是生物分离分析中发挥的作用日益增大。整体柱的制备及应用近年来也得到了快速发展，层出不穷的新型整体柱已被广泛用于色谱高效分离分析、固相萃取及酶反应器等方面，大大推动了分离分析科学的发展。本文主要总结了近五年来整体柱的制备技术及其在蛋白质组学应用中的一些最新研究进展。
英文摘要：
Monolithic column is a continuous unitary porous structure prepared by in-situ polymerization or consolidation inside the column tubing. Monolithic columns have been attracted great attention due to their simple preparation procedure, high permeability and high mass transfer rate. Big progress on the technology and methods for the preparation of monolithic columns and their applications to the highly efficient separation and pretreatment of biological samples have been made in last decade. This review summarizes the developments in the preparation of the monolithic columns and their applications to the proteomic analysis during the last five years
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Highly specific enrichment of phosphopeptides by zirconium dioxide nanoparticles for phosphoproteome
Polyhedral Oligomeric Silsesquioxane as a Cross-linker for Preparation of Inorganic-Organic Hybrid M
Combined Comparative and Chemical Proteomics on the Mechanisms of levo-Tetrahydropalmatine-Induced A
The synthesis of chloropropyl-functionalized silica hybrid monolithic column with modification of N,
Identification of Angiotensin I-Converting Enzyme Inhibitors in Peptides Mixture of Hydrolyzed Red D
Fractionation of phosphopeptides on strong anion-exchange capillary trap column for large-scale phos
Classification Filtering Strategy to Improve the Coverage and Sensitivity of Phosphoproteome Analysi
Reversed-Phase-Reversed-Phase Liquid Chromatography Approach with High Orthogonality for Multidimens
Coupling Strong Anion-Exchange Monolithic Capillary with MALDI-TOF MS for Sensitive Detection of Pho
Phosphoproteome analysis of human liver tissue by long-gradient nanoflow LC coupled with multiple st
P-Glycoprotein Antibody Functionalized Carbon Nanotube Overcomes the Multidrug Resistance of Human L
Vinyl functionalized silica hybrid monolith-based trypsin microreactor for on line digestion and sep
Selective extraction of peptides from human plasma by highly ordered mesoporous silica particles for
Preparation of Perphenylcarbamoylated beta-Cyclodextrin-silica Hybrid Monolithic Column with "One-Po
Development of Glycoprotein Capture-Based Label-Free Method for the High-throughput Screening of Dif
The highly selective capture of phosphopeptides by zirconium phosphonate-modified magnetic nanoparti
Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosph
Online multidimensional separation with biphasic monolithic capillary column for shotgun proteome an
Profiling of Endogenous Serum Phosphorylated Peptides by Titanium (IV) Immobilized Mesoporous Silica
Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidom
Phosphonic Acid Functionalized Periodic Mesoporous Organosilicas and Their Potential Applications in
Selective on-line serum peptide extraction and multidimensional separation by coupling a restricted-
Comprehensive two-dimensional high-performance liquid chromatography system with immobilized liposom
Evaluation of the interaction of bioactive compounds in Cortex Pseudolarix and Radix Stephaniae by t
Comprehensive and Reliable Phosphorylation Site Mapping of Individual Phosphoproteins by Combination
Glycoproteomics Analysis of Human Liver Tissue by Combination of Multiple Enzyme Digestion and Hydra
Integration of monolithic frit into the particulate capillary (IMFPC) column in shotgun proteome ana
Preparation of monodisperse immobilized Ti4+ affinity chromatography microspheres for specific enric
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