研究对采自青岛沿海的两种海洋纤毛虫-盾圆双眉虫与伪寡毛双眉虫做了形态学重描述。盾圆双眉虫与前人所报道的种群具有十分相似的纤毛图式,但在额-腹棘毛分布、大核片段、小膜及背触毛数目等方面表现出细微的变异性。此外,该青岛种群个体较小。统计学比较还表明,迄今缺乏研究的一海洋种,泥生双眉虫极可能为盾圆双眉虫(Diophrys scutum)的同物异名。伪寡毛双眉虫(Diophrys apoligothrix)为一新近报道的罕见种,研究基于新采集种群对其进行了补充性观察和描述。
Diophrys species are a group of marine cosmopolitan free-living ciliates,therefore there are many reports concerning their morphology and diversity.However,species identification remains problematic owing to the fact that many previous descriptions were basically based on live observation and morphometric,and infraciliature data still re-main lacking for some known species.The aims of this paper were to supply new information and to clarify confusions of two known Diophrys species,D.scutum(Dujardin,1841) Kahl,1932 and D.apoligothrix Song et al,2009.Both organisms were collected from the upper layer of sandy sediments in the intertidal region in Qingdao,China,where the water temperature was about 18℃ and the salinity was 18‰.After isolation,pure cultures were kept,to which some rice grains were added to enrich bacterial food.Ciliates were examined in vivo using bright field and differential interference contrast microscopy.The infraciliature was revealed with protargol staining method.Counts and measurements of stained specimens were performed at a magnification of 1250 ×.Based on present and previous studies,an improved diagnosis was presented here for D.scutum: marine Diophrys,broadly oval body outline,in vivo 80-200 μm long;43-80 adoral membranelles and the distal end of adoral zone was strongly curved;5-8 frontoventral,5-6 transverse,2-3 left marginal and 3-4 caudal cirri;5-9 dorsal kineties,which were always densely ciliated;usually two(rarely one) sausage-shaped macronuclear nodules.D.apoligothrix was a rare species,and the current population of it was morphologically identified by:(35-75) μm ×(25-40) μm in vivo;body rigid with sculptured surface;adoral zone comprising about 24-31 membranelles;5 frontal,2 ventral,5 transverse,1 left marginal and 3 caudal cirri;5 dorsal kineties and the middle kinety had about 5-6 basal body pairs,dorsal cilia about 5-10 μm;usually two ellipsoid macronuclear nodules;marine habitat.By now,nine morphospecies were included in the genus Diophrys,name