在高凝油藏开发过程中,原油易在孔隙中析蜡、发生胶质和沥青质沉积等。研究不同水驱环境中内源微生物群落及其与高凝油的相互作用关系,以河南魏岗油田高凝油藏产出水样为研究对象,优选最佳激活体系;通过微生物驱油物理模拟实验考察内源微生物提高采收率情况,研究激活前后内源微生物的群落变化以及原油组分、性质的变化。结果表明:注采水样中微生物群落差异明显,内源微生物被激活后优势菌为芽胞杆菌(Bacillus sp)和梭菌(Clostridium ultunense)等,原油乳化分散等级最高可达5级,微生物提高采收率最高为12.51%;凝点最多降低5℃,蜡含量降低3.95%以上,同时多环芳烃萘、菲及其同系物等降解较为明显;不同水驱环境中内源微生物在驱油、原油降解等过程中发挥着重要的作用。
The wax, resin, and asphaltene in the high-wax crude oil can be easily precipitated in the pores during the devel- opment of this crude oil. The interaction between the indigenous microorganisms in different water floodings and high-wax crude oil was investigated. The injection and production water was collected from Weigang Oilfield (Henan) and the activa- tion system was optimized. The oil recovery enhancement with the indigenous microbe was studied using physical simulation experiment of oil displacement by microorganism. And the changes of the microbial community, as well as the composition and properties of crude oil before and after activation were characterized. It is found that the difference of the microbial com- munity in injection and production water is significant. The dominant bacteria after activation in indigenous microbe are Ba- cillus sp and Clostridium ultunense. The dispersion grade of the oil emulsion is reached up to 5. And the enhanced oil recov- ery by microbe is increased up to 12. 51%. The freezing point of the oil is reduced down to 5 ℃, and the wax content is de- creased more than 3.95 %. Meanwhile, the degradation of naphthalene, phenanthrene and its homologue in crude oil is pronounced. The indigenous microbes in different water floodings play an important role of oil recovery and oil degradation.