中文摘要：

通过比较野生型、Asch9和Asch9（SCH9—3HA）三种酵母菌种在葡萄糖、半乳糖、蔗糖和麦芽糖作碳源培养基上的生长表型研究SCH9是否参与酵母不同碳源代谢利用调控．结果显示，Asch9细胞菌落大小较野生型细胞小且有明显的生长缺陷，但这种生长缺陷在重新获得sch-3HA基因后得到恢复．通过比较野生型、Asch9和Asch9（SCH9—3HA）三种酵母菌种在渗透压胁迫和热胁迫条件下的生长表型发现sch9可能参与了酵母胁迫应答．利用抗SCH9570位磷酸化苏氨酸特异性抗体（anti—T570一P），通过变性免疫沉淀和免疫印迹方法，研究了生理条件下的Asch9（SCH9—3HA）细胞裂解液中SCH9激活环的磷酸化状态．结果显示，在生理条件下SCH9激活环T570位点发生了明显磷酸化．进一步研究了渗透压胁迫条件下SCH9激活环T570位点的磷酸化水平．结果表明，渗透压胁迫条件下SCH9激活环T570位点磷酸化水平较生理条件下显著增强．结果显示SCH9可能通过增强激活环磷酸化水平来参与调控酵母不同碳源代谢和胁迫应答．

英文摘要：

The phenotypes of three yeast strains wildtype, Asch9 and Asch9 （SCH9-3HA） growing on different carbon sources containing media were studied. The results showed that Asch9 cell colony was smaller than that of wildtype and had obvious growth defects, but which can be restored when SCHg- 3HA gene was transformed into Asch9 cell. Through comparing the phenotypes of three strains wild- type, Asch9 and Asch9（SCHg-3HA） under osmotic and thermal stress, the observers found that SCH9 probably is involved in the regulation of stress response. Using specific for phosphorylated 570 threonine （T570） of SCH9 antibody （anti-T570-P）, denaturing immunoprecipitation and immunoblot methods, SCH9 activation loop phosphorylation under physiological condition was studied. The results showed that SCH9 activation loop T570 site phosphorylated evidently under physiological condition. The phosphorylation status of SCH9 activation loop T570 site under osmotic stress condition was further studied. The results showed that SCH9 activation loop T570 site phosphorylation intensity was increased evident- ly. All results indicated that SCH9 probably regulate different carbon sources metabolism and stress re- sponse through increasing its activation loop phosphorylation intensity.