中文摘要：

【目的】克隆龙眼（Dimocarpus longan Lour.）胚性愈伤组织线粒体ATP合酶β亚基基因（mitochondrial F1-ATPase beta subunit gene）,并分析该基因在龙眼体胚发生过程中的表达情况。【方法】采用RT-PCR结合RACE法,通过T/A克隆测序,获得龙眼胚性愈伤组织线粒体ATP合酶β亚基基因全长序列;随后通过实时荧光定量PCR（qRT-PCR）法研究该基因在龙眼体胚发生过程中的表达规律。【结果】成功克隆龙眼胚性愈伤组织线粒体ATP合酶β亚基基因完整cDNA序列（GenBank登录号：FJ222749）,该序列全长2099bp,由1677bp核苷酸组成的ORF,编码558个氨基酸。该基因与其它植物的线粒体ATP合酶β亚基基因在核苷酸序列和推导的氨基酸序列方面相似性较高。对来源于动、植物的28条线粒体ATP合酶β亚基基因编码区序列所构建的进化树分析表明,由线粒体ATP合酶β亚基基因编码区序列所建立的系统关系树与真实的动、植物进化基本一致,龙眼处在双子叶植物中,由于该基因在龙眼同科属的植物中为首次克隆,所以有自己单独的分支。qRT-PCR结果分析表明,随着龙眼体胚的发育,线粒体ATP合酶β亚基基因转录水平逐渐升高,到球形胚阶段达到最高,而后又急剧下降,到鱼雷形胚阶段降到最低,子叶形胚阶段略有升高。【结论】龙眼线粒体ATP合酶β亚基基因与其它植物相应序列具有较高同源性,在龙眼体胚发育过程中,以球形胚阶段的表达最高。

英文摘要：

【Objective】 The mitochondrial F1-ATPase beta subunit gene from embryogenic callus was cloned and its expression during somatic embryogenesis in longan （Dimocarpus longan Lour.） was analyzed in this experiment. 【Method】 The complete cDNA sequence of the mitochondrial F1-ATPase beta subunit gene of longan embryogenic callus was obtained by reverse transcription polymerase chain reaction （RT-PCR） with rapid amplification of cDNA ends （RACE）. And the mRNA transcription level of this gene was surveyed by real-time reverse transcription PCR （qRT-PCR） during somatic embryogenesis in longan. 【Result】 The full-length cDNA sequence of longan embryogenic callus mitochondrial F1-ATPase beta subunit gene was 2 099 bp （GenBank accession number FJ222749）. It contained a 1 677-nucleotides-long open reading frame （ORF） which encoded a protein of 588 amino acid residues. Homology searches with the deduced amino acid residues indicated that longan mitochondrial F1-ATPase beta subunit gene had a high similarity to other plant mitochondrial F1-ATPase beta subunit gene. According to the 28 mitochondrial F1-ATPase beta subunit gene sequences of animals and plants,the phylogenetic tree showed that it indeed reflected the evolution of animals and plants. It had a single branch because longan mitochondrial F1-ATPase beta subunit gene was firstly cloned. The results showed that the mRNA transcription levels of longan mitochondrial F1-ATPase beta subunit gene were different at the different stages during longan somatic embryogenesis. The highest level occurred at the globular embryo stage and the lowest at the torpedo embryo stage. 【Conclusion】 Longan mitochondrial F1-ATPase beta subunit gene had a high similarity to other plant mitochondrial F1-ATPase beta subunit gene. The highest mRNA transcription level of longan mitochondrial F1-ATPase beta subunit gene occurred at the globular embryo stage.