中文摘要：

目的：探讨斑蝥酸钠维生素B6（sodiumcantharidinateandvitaminB6，SCAVB6）对人肝癌SMMC-7721细胞增殖、凋亡和侵袭及乙酰肝素酶（heparanase，HPA）表达的影响。方法：0．5、1．0、1．5和2．0gg／mLSCAVB6作用肝癌SMMC-7721细胞24、48和72h后，应用MTT法检测细胞的增殖情况。0．5、1．0、1．5和2．0gg／mLSCAVB6作用肝癌SMMC-7721细胞48h后，FCM法检测细胞的凋亡情况，Transwell侵袭实验检测细胞的侵袭能力，RT—PCR和蛋白质印迹法检测细胞中HPAmRNA和蛋白的表达水平。结果：SCAVB6能够抑制SMMC-7721细胞的增殖，且具有-定的浓度和时间依赖性（p〈0．05）。SCAVB6作用48h后，肝癌SMMC-7721细胞的凋亡率高于对照组（SMMC-7721细胞未进行任何干预），而侵袭能力低于对照组（P值均〈0．05）。1．0、1．5和2．0gg／mLSCAVB6作用后，SMMC-7721细胞中HPAmRNA和蛋白的表达水平低于对照组，且呈浓度依赖性（P值均〈0．05）。结论：SCAvB6可抑制SMMC-7721细胞的增殖和侵袭，这-作用可能与抑制HPA的表达有关。

英文摘要：

Objective： To investigate the effects of sodium cantharidinate and vitamin B6 （SCAVB6） on cell proliferation, apoptosis and invasion of hepatic cancer cell line SMMC-7721 as well as the expression of heparanase （HPA）. Methods： After treatment with 0.5, 1.0, 1.5 and 2.0 μg/mL SCAVB6 for 24, 48 and 72 h, the cell proliferation was detected by MT＋ assay. The cell apoptosis, invasion ability and the expression levels of HPA mRNA and protein of SMMC-7721 cells after treatment with 0.5, 1.0, 1.5 and 2.0 μg/mL SCAVB6 for 48 h were examined by flow cytometry（FCM）, Transwell cell invasion assay, RT-PCR and Western blotting, respectively Results： SCAVB6 could inhibit the proliferation of SMMC-7721 cells in a dose- and time- dependent manner （P 〈 0.05）. The apoptotic rate of SMMC-7721 cells after treatment with SCAVB6 for 48 h was higher than that of the SMMC-7721 cells without any treatment, but the invasion ability was weakened （both P 〈 0.05）. The expression levels of HPA mRNA and protein in SMMC-7721 cells after treatment with 1.0, 1.5 and 2.0 μg/mL SCAVB6 were lower than those in the SMMC-7721 cells without any treatment, and this inhibition effect was in a dose-dependent manner （all P 〈 0.05）. Conclusion： SCAVB6 can inhibit the proliferation and invasion of SMMC-7721 cells. This effect may be related to the inhibition of expression of HPA.