中文摘要：

对大花杓兰（Cypripedium macranthum Sw.）内生真菌类群组成及多样性进行了分析。结果表明,从大花杓兰植株中分离到内生真菌59株,利用ITS-r DNA序列分子鉴定法将大花杓兰内生真菌鉴定为18个分类单元,分布在7纲9目11科16属。北京大花杓兰内生真菌包括12个属,内生真菌的多样性指数H=1.39、D=0.96,优势菌群为毛壳菌属（Chaetomium）和茎点霉属（Phoma）真菌,分别占总菌株数的15.25%和8.47%;吉林大花杓兰样品内生真菌归于9个属,内生真菌的多样性指数H=1.31、D=0.93,以毛壳菌属（Chaetomium）为优势菌群,占总菌株数的23.73%。由此可见,北京大花杓兰中内生真菌多样性高于吉林大花杓兰。

英文摘要：

The composition and diversity of endophytic fungal taxa in Cypripedium macranthum Sw was analyzed. The results showed that a total of 59 fungal strains were isolated from C. macranthum. These fungi were belonged to 18 taxa containing 7 classes, 9 orders, 11 families and 16 generas with analyzing ITS-rDNA sequence. Endophytic fungal groups in Beijing C. Macranthum included to 12 generas, with the diversity index H of 1.39 and D of 0.96. Chaetomium （15.25%） and Phoma （8.47%） were two major fungal groups. Endophytic fungal groups in Jilin C. Macranthum included 9 generas, with the diversity index H of 1.31 and D of 0.93.Chaetomium belonged to the core group（23.73%）. Diversity of endophytic fungi in Beijing C. Macranthum was higher than that of Jilin C. Macranthum.