中文摘要：

目的：研究肝郁脾虚证胃溃疡大鼠经痛泻要方治疗后胃窦组织蛋白质组学的变化,探讨肝郁脾虚证胃溃疡发病及痛泻要方可能的作用机制。方法：建立阿司匹林诱发大鼠胃溃疡及多种不同应激方法进行情志刺激引起的大鼠肝郁脾虚证模型,通过痛泻要方动物体内给药,研究其对于大鼠一般体征、溃疡指数、差异蛋白表达的影响。采用黏膜损伤评分法研究痛泻要方对大鼠溃疡指数的影响;采用大鼠胃窦组织样本i TRAQ蛋白质组学检测方法,探寻差异蛋白表达。结果：痛泻要方能改善胃溃疡大鼠一般体征,明显减轻胃溃疡大鼠的溃疡指数并明显减轻胃溃疡大鼠的胃液酸度,差异蛋白筛选、Gene ontology分析、差异蛋白pathway分析及基因网络分析表明,痛泻要方对肝郁脾虚证胃溃疡胃窦部差异蛋白及差异基因的表达水平有明显的修复作用,痛泻要方对肝郁脾虚证大鼠有机物代谢、大分子代谢、初级代谢、细胞代谢过程、单有机体代谢过程有明显的调控作用,并对Kit受体信号通路、蛋白酶体降解、细胞因子及炎症反应、IL-5信号通路、Omega脂肪酸氧化、MAPK信号通路、mRNA加工、补体活化途径、炎症反应通路、Statin通路、IL-3信号通路、凝血通路、条纹肌收缩、TNF-α及NF-κB信号通路、EGFR1信号通路、胰岛素信号通路、补体和凝血级联反应通路、胞浆核糖体蛋白通路等炎症通路、免疫调节通路、细胞增殖及凋亡通路及分子具有广泛的调节作用。结论：痛泻要方对阿司匹林及多种不同应激方法进行情志刺激引起的肝郁脾虚证胃溃疡模型具有明显的治疗作用,肝郁脾虚证胃溃疡发病会导致胃窦组织蛋白质组学的显著变化,而痛泻要方对差异蛋白及信号通路具有显著的修复调节作用。

英文摘要：

Objective：To investigate the gastric antrum proteomics of Tongxie Yaofang on syndrome of liver qi stagnation and spleen deficiency in rats. Methods：The syndrome of liver qi stagnation and spleen deficiency in rats was established by aspirin-induced acute gastricmucosa injury which followed by multiple emotional stimulation stress. General physical signs, ulcer index and difference protein expression were studied while the rats were intragastric administration with Tongxie Yaofang. Mucosal injury score was used to evaluate the ulcer index of the Tongxie Yaofang. iTRAQ proteomics analysis on gastric antrum tissue was used to study the protein expression difference. Results：Tongxie Yaofang could improve the general physical signs of gastric ulcer rats, and reduce the ulcer inder and gustric juice acidity significantly, iTRAQ proteomics analysis of difference proteins, gene ontology, pathways and gene network results indicated that Tongxie Yaofang had obvious recovery effect on differece gene and protein expression of the gastric ulcer rats, and had obvious regulation effect on organic substance metabolic process, macromolecule metabolic process, primary metabolic process, cellular meta- bolic process and single-organism metabolic process,also had broaden adjust effect on a couple of signal pathways covering inflammation,immuno-regulation and cell proliferation and apoptosis such as Kit receptor signaling pathway, proteasome degradation, cytokines and inflammatory response, IL-5 signaling pathway, fatty acid omega oxidation, MAPK signaling pathway, mRNA processing, complement activation classical pathway, inflammatory response pathway, statin pathway, IL-3 signaling pathway, blood clotting cascade, striated mus- cle contraction, TNF-α NF-κB signaling pathway, EGFRl signaling pathway, insulin signaling, cytoplasmic ribosomal proteins pathway. Conclusion ： Tongxie Yaofang has obvious therapeutic effect on syndrome of liver qi stagnation and spleen deficiency in rats, in which the proteomicsaltered significant