中文摘要：

目的：／A基因组水平揭示冶金微生物喜温嗜酸硫杆菌SM-1的重金属抗性分子机制。方法：采用454GSFLX测序平台对SM-1进行基因组测序，利用NCBI非冗余蛋白数据库、Uniport蛋白数据库以及基因组百科全书数据库（KEGG）对全基因组序列进行功能注释，通过比较基因组分析参与重金属抗性的基因及可能的分子机制。结果：基因组中34个基因编码重金属抗性相关蛋白，其中29个基因参与砷酸盐、汞、镉、锌、钴等重金属抗性机制；38个基因参与维持细胞内适宜的蛋白质折叠环境，这将有助于SM-1适应高浓度重金属离子的生物冶金环境。结论：基因组学手段为全面揭示喜温嗜酸硫杆菌的重金属抗性分子机制提供了帮助，也为进一步通过遗传改造提升其重金属抗性、进而提高冶金效率提供理论指导。

英文摘要：

Objective：The genomic method was utilized to discover the molecular mechanisms of heavy metal resistance of Acidithiobacillus caldus SM - 1. Method： Genomic DNA of At. caldus SM - 1 was sequenced by the Roche 454 Genome Sequencer FLX instrument. Genefunction was annotated by homology searching in the NCBI NR（ non -redundant）, UniProt and KEGG protein database. Genes related to heavy metal resistance were identified by the comparative genomic analysis. Result ：34 genes involved in heavy metal resistance were iden- tiffed in the genome of SM - 1,29 of which were responsible for the arsenic, fiaercury, cadmium, cobalt, zinc resistance. 38 genes were enco- ded to maintain an appropriate environment for protein folding, which will help for cell adaptation to the bioleaching environment. Conclusion： Genomics provides an novel strategy to unveil the genetic bases of AcidithiobaciUus caldus SM - 1 heavy metal resistance,which will further give insights to construct the engineered stains with better bioleaching performance.