中文摘要：

对小麦RAPD常规的扩增循环参数及琼脂糖浓度进行了试验优化。结果表明：适宜的扩增循环参数为：94℃预变性2min，94℃15s→36℃15s→72℃30s40个扩增循环，72℃延伸10min，4℃保持。用于电泳介质的琼脂糖最佳浓度为2％。时间比常规减少一半。

英文摘要：

This paper involved in Optimization for Circular Parameter and Agarose Concentration of RAPD in Wheat. The circular parameter of RAPD was 94℃ 2 min predenaturation,2min,94℃15s→36℃15s→72℃30s by 40 cycling,72 ℃ 10 min extending,4 ℃ holding. As media of electrophoresis, optimum concentration of agarose was 2%. The time has decreased by one-second.