在常温条件下使用超高压技术提取北虫草多糖,采用正交试验设计对提取工艺条件进行优化。结果表明最优提取工艺条件为溶剂45%乙醇、压力400MPa、料液比1:35(g/ml)、提取时间5min,此条件下北虫草多糖提取率为10.13%。该方法不仅可以快速高效地提取出有效成分,而且保持其生理活性,为天然产物有效成分的提取提供了一种崭新的提取方法。
Ultra high pressure (UHP) technology was adopted to extract polysaccharides from Cordyceps militaris (L.ex Ft.) Link at normal temperature. The extraction of polysaccarides by UHP technology was optimized to obtain the maximum yield of polysaccarides by single-factor method and orthogonal array design. A polysaccharide yield of 10.13% was found under the optimized conditions for the extraction of polysaccarides from Cordyceps militaris (L.ex Fr.) Link as follows: extraction for 5 min using 45% as the extraction solvent at 400 MPa with the material/liquid ratio of 1:35 (g/ml). Polysacchaddes can be extracted rapidly and effectively by this method without losing their physiological activity, thereby providing a novel and robust approach for extracting active components from Cordyceps militaris (L.ex Ft.) Link.