中文摘要：

【目的】采集板栗园中板栗外生菌根，对其进行真菌的分离、鉴定，并与植物病原菌进行拮抗试验，筛选出具有生防作用的真菌。【方法】以板栗外生菌根为材料筛选单菌落真菌，并利用真菌核糖体RNA内转录间隔区（ITS）序列的同源性进行分子鉴定。筛选长势良好的真菌与15种植物病原菌进行四点对峙试验。【结果】从板栗菌根中分离并鉴定了螺旋木霉、钩状木霉、生赤壳等15个属的20个菌种。通过四点对峙试验发现，螺旋木霉和钩状木霉拮抗病原菌种类在13种以上，具有广谱性。【结论】螺旋木霉和钩状木霉拮抗效果较好，可作为候选菌种进行生防菌剂生产。

英文摘要：

[[Objective]In order to select out beneficial fungi with broad-spectrum antagonism, the fungi from Chinese chestnut ectomycorrhiza which were collected from chestnut orchard were isolated and identified , and then the fungi were used to study the antagonistic effects with plant pathogen fungi. [Methods]Pure culture fungal strains were isolated from the chestnut ecto- mycorrhiza and identified by the homology of Internal transcribed spacer （ITS） sequence of the fungal ribosomal RNA. Then well-growing fungi were used for luther antagonistic cultivation with 15 pathogens through co culture method. [Results]Total 20 fungal strains were isolated from chestnut roots, including Trichoderma spp.,Phornopsis spp., Cyli71drocarpon spp., and so on. The antagonistic cultivation analysis showed two strains from genus Trichoderma had broad-spectrum antagonistic effect against 13 pathogens species. [Conclusion] Due to the well broad antagonistic effect, the fungi of genus Trichoderma could be the candidate strains for further development and application of bio-control agents.