中文摘要：

目的 探讨高脂饮食饲养对小鼠前额皮层神经细胞线粒体DNA/mRNA稳定性的影响. 方法 雄性小鼠40只按随机数字表法分为正常饮食组20只、高脂饮食组20只,各组饲养4个月、6个月后处死（各10只）.应用常规试剂盒检测各时间点小鼠血清血糖、血脂及胆固醇水平变化;分离纯化神经细胞线粒体,利用普通PCR及实时定量PCR技术检测线粒体DNA/mRNA的变化. 结果 4个月时高脂饮食组小鼠血糖、血脂和胆固醇较正常饮食组差异无统计学意义（P＞0.05）,6个月时高脂饮食组小鼠血脂水平较正常饮食组明显升高,差异有统计学意义（P＜0.05）.4个月、6个月时高脂饮食组小鼠D-Loop水平与正常饮食组比较差异均无统计学意义（P＞0.05）.与正常饮食组比较,6个月时高脂饮食组小鼠ATPase6水平降低,Cyt3和Cytb水平升高,差异有统计学意义（P＜0.05）. 结论 持续高脂饮食饲养6个月后小鼠神经细胞线粒体基因稳定性下降,首先是线粒体RNA表达模式异常.

英文摘要：

Objective To investigate the effects of high fat diet on stability of mitochondrial DNA/mRNA of frontal cortex tissue in mice.Methods Forty male mice were randomly divided into regular diet group and high fat diet group （n=20）.All animals were sacrificed at 4 months （n=10） or 6 months （n=10） after experiment.The serum glucose,lipid and cholesterol levels were evaluated.And mitochondria were isolated in the frontal cortex of mice,and the changes ofmitochondrial DNA/mRNA were tested using PCR and real-time quantitative PCR.Results The serum glucose,lipid and cholesterol levels showed no significant changes in the mice of high fat diet group after feeding for 4 months as compared with those in the mice of regular diet group （P〉0.05）,while the lipid level in the high fat diet group was increased significantly as compared with that in the regular diet group after feeding for 6 months （P〈0.05）.The D-Loop level showed no significant changes after feeding for 4 and 6 months between the high fat diet group and the regular diet group （P〉0.05）.Decreased ATPase6 and increasd Cyt3 and Cytb expression levels in the high fat diet group were noted as compared with those in the regular diet group after feeding for 6 months（P〈0.05）.Conclusion The stability of mitochondrial DNA decreases in the brain of mice at the persistent feeding with high fat diet for 6 months; especially,pattem of mitochondrial mRNA expression changes obviously at early stage.