中文摘要：

【目的】克隆鲢胰岛素样生长因子结合蛋白1（IGFBP-1）基因,分析其编码蛋白的结构与功能,并分析低氧胁迫下该基因在鲢肝脏中的表达情况。【方法】采用cDNA末端快速扩增技术（Rapid amplification of cDNAends,RACE）扩增鲢IGFBP-1基因的全长cDNA,通过半定量RT-PCR法对鲢肌肉、心脏、脑、肾脏、肝脏、腮和脾等组织器官IGFBP-1mRNA的表达水平进行检测,利用Real-time PCR法检测急性低氧胁迫0,2,4,6,8,10和12h后鲢肝脏组织中IGFBP-1表达量的变化。【结果】鲢IGFBP-1全长cDNA为1 081bp,具有73bp的5′非编码区（Un-translated Regions,UTR）、789bp的开放读码框（Open reading frame,ORF）,以及219bp的3′UTR,编码含262个氨基酸的蛋白质。RT-PCR结果表明,IGFBP-1在肌肉、心脏、脑、肾脏、肝脏、腮和脾等7种组织中均有表达,其中在肝脏中的表达量最高,心脏和肌肉次之,在脑中的表达量最低;Real-time PCR结果表明,在低氧胁迫6h时,IGFBP-1cDNA在肝脏中的表达量显著增高,随后略有降低但仍显著高于未进行低氧胁迫处理组。【结论】克隆得到鲢IG-FBP-1全长cDNA,该基因在肝脏组织中大量表达;随着低氧胁迫时间的延长,鲢肝脏中IGFBP-1mRNA表达量升高,达最大值后继续低氧胁迫其表达量略有降低。

英文摘要：

【Objective】 The study aimed to clone the cDNA of IGFBP-1 and analyze the encoded amino acids and the expression level under hypoxia in liver.【Method】 The method of Rapid amplication of cDNA ends（RACE）was used to obtain the complete IGFBP-1 cDNA sequence.Semi-quantitative RT-PCR was performed using the tissues of muscle,heart,brain,kidney,liver,gill and spleen of silver carp.The expression patterns of IGFBP-1 under the condition of acute hypoxia for 0,2,4,6,8,10 and 12 h was analyzed by real-time PCR.【Result】 The full-length cDNA of IGFBP-1 was 1 081 bp and the open reading frame was 789 nucleotides flanked by a 73 nucleotides of 5’-UTR and a 219 nucleotides of 3’-UTR.The ORF encoded a predicted polypeptide of 262 amino acids.RT-PCR showed that IGFBP-1 mRNA was expressed in muscle,heart,brain,kidney,liver,gill and spleen and showed high expression in liver,heart and muscle and low expression in brain.Real-time PCR revealed that in liver,the expression level increased significantly at 6 hours under hypoxia,and decreased a little after then but kept at a high level compared to the control group.【Conclusion】 A full-length cDNA of IGFBP-1 was cloned.IGFBP-1 mRNA was abundantly expressed in liver and the expression level increased after acute hypoxia.The peak was at 6 h after hypoxia and it slightly decreased after the peak.