中文摘要：

棉花纤维开发和体的胚胎开始的机制与微数组和抑制系统地被探索了减少性的杂交。实时 RT-PCR 在许多不同样品，从微数组或其它的数据能详细与被证实提供基因表示的同时的测量。为了完成精确、可靠的基因表示，结果，对一个或几内部控制基因的即时 PCR 数据的正规化被要求，它不应该在开发的各种各样的阶段期间在不同纸巾波动。我们估计了 7 经常使用的家务基因的基因表示，包括 18S rRNA， HistoneS， UBQ7，肌动朊， Cyclophilin， Gbpolyubiquitin-1 和 Gbpolyubiquitin-2，在 21 件棉花样品的一个多样的集合。为纤维发展系列所有家务基因的表示在 17 DPA 以后在趋势下面有一样。但是在以后的纤维发展阶段有高表示水平的 AGP 基因(arabinogalactan 蛋白质) 的表示从 15 ～ 27 DPA 是起来调整的。相对绝对数量化离子应该因此是为纤维的一个有效、方便的方法发展系列。表示非，纤维纸巾系列对纤维不如此变化了发展系列。并且三最好控制基因 HistoneS， UBQTand Gbpolyubiquitin-1 不得不以得到更好的正规化的一个 combinated 方法被过去常。

英文摘要：

The mechanisms of cotton fiber development and somatic embryogenesis have been explored systematically with microarray and suppression subtractive hybridization. Real-time RT-PCR provides the simultaneous measurement of gene expression in many different samples, with which the data from microarray or others can be confirmed in detail. To achieve accurate and reliable gene expression results, normalization of real-time PCR data against one or several internal control genes is required, which should not fluctuate in different tissues during various stages of development. We assessed the gene expression of 7 frequently used housekeeping genes, including 18S rRNA, Histone3, UBQ7, Actin, Cyclophilin, Gbpolyubiquitin-1 and Gbpolyubiquitin-2, in a diverse set of 21 cotton samples. For fiber developmental series the expression of all housekeeping genes had the same down tendency after 17 DPA. But the expression of the AGP gene （arabinogalactan protein） that has high expression level at the later fiber development stage was up-regulated from 15 to 27 DPA. So the relative absolute quantification should be an efficient and convenient method for the fiber developmental series. The expression of nonfiber tissues series varied not so much against the fiber developmental series. And three best control genes Histone3, UBQ7 and Gbpolyubiquitin-1 have to be used in a combinated way to get better normalization.