中文摘要：

通过捕获探针与纳米金膜之间的共价连接，保证了滚环扩增（ RCA）产物始终结合于金膜表面， Phi29 DNA聚合酶的高效扩增和Escherichia coli DNA链接酶的高度精确性使检测达到单碱基识别，检测灵敏度达到104 copies/mL.实验结果表明，与单碱基错配序列相比， RCA可明显增强检测的灵敏度.该RCA基因传感器操作简单，灵敏度和特异性较高，在乙型肝炎病毒的快速检测方面具有一定的开发潜力.

英文摘要：

The research describes the application of RCA（ rolling circle amplification）-based quartz crystal microbalance（ QCM） biosensor for the detection of hepatitis B virus（ HBV） DNA. RCA is an isothermal am-plification technique, which creats long single-stranded products with property of product localization. After amplification, the RCA product is maintained during the assay through the covalent bonding between the cap-ture probes and the gold electrode surface. Using high amplification efficiency of Phi29 DNA polymerase and remarkable precision of Escherichia coli DNA ligase, the detection limit can reach 104 copies/mL. Experimen-tal results show that RCA has significantly enhanced sensitivity for the target strand compared to the single-base mismatch strand. RCA has powerful amplification ability, and QCM has superb mass sensitivity. The combination of these two methods provides a high sensitive RCA-QCM biosensor method, which has the poten-tial to become a successful clinical application.