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Cloning and Identification of Novel MicroRNAs from Rat Hippocampus
  • ISSN号:1672-9145
  • 期刊名称:《生物化学与生物物理学报:英文版》
  • 分类:Q78[生物学—分子生物学]
  • 作者机构:[1]Institute of Clinical Molecular Biology, Peking University People's Hospital, Beijing 100044, China
  • 相关基金:This work was supported by a grant from the National Natural Science Foundation of China (No. 30540033)
中文摘要:

MicroRNAs (miRNAs ) 是小规章的分子 post-transcriptionallysuppressingmRNA 活动。在各种各样的有机体的许多 miRNAs 被克隆但是许多未知 miRNAs 遗体到我们描述从老鼠马头鱼尾的怪兽克隆六新 miRNAs 的 beidentified.Here。在他们之中,四没在老鼠米尔底被发现,但是与他们的人或老鼠相当或相同的事物相同,因此,他们被指定为 rno-miR-92b , rno-miR-146b , rno-let-7g ,和 rno-miR-551b.The 另外的二从 rno-miR-330 和 rno-miR-384 的已知的 miRNA 先锋的另外的手臂被导出,并且没在所有有机体的米尔底被发现。他们被指定为 rno-miR-330 * 并且这些 miRNAs 的 rno-miR-384 *.The 表示被跟踪 RNA 和教材扩展证实即时 reversetranscription 聚合酶链反应。这六 miRNAs 被表示在在马头鱼尾的怪兽显著地高级比在另外的纸巾包括服的外皮,心,肝,肺 andkidney.miR-384 * 是比在老鼠马头鱼尾的怪兽的 miR-384 更丰富的 10 次,但是小差别在 miR-330 之间被发现 * 并且在一样的织物的 miR-330 表示。

英文摘要:

MicroRNAs (miRNAs) are small regulatory molecules post-transcriptionally suppressing mRNA activity. Many miRNAs in various organisms have been cloned but many unknown miRNAs remain to be identified. Here we describe the cloning of six new miRNAs from rat hippocampus. Among them, four were not found in the rat miRBase, but were identical to their human and/or mouse homolog, therefore they were designated as rno-miR-92b, rno-miR-146b, rno-let-7g, and rno-miR-55 lb. The other two were derived from the other arms of the known miRNA precursors of rno-miR-330 and rno-miR-384, and were not found in miRBase of all organisms. They were designated as rno-miR-330^* and rno-miR-384^*. The expression of these miRNAs was confirmed by RNA-tailing and primer-extension real-time reverse transcriptionpolymerase chain reaction. These six miRNAs were expressed at significantly higher levels in the hippocampus than in other tissues, including cerebral cortex, heart, liver, lung and kidney, miR-384^* was 10 times more abundant than miR-384 in rat hippocampus, but little difference was found between miR-330^* and miR-330 expression in the same tissue.

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期刊信息
  • 《生物化学与生物物理学报:英文版》
  • 北大核心期刊(2004版)
  • 主管单位:
  • 主办单位:中国科学院上海生物化学研究所
  • 主编:
  • 地址:上海岳阳路319号
  • 邮编:200031
  • 邮箱:abbs@sibs.ac.cn
  • 电话:021-54920956 54920955
  • 国际标准刊号:ISSN:1672-9145
  • 国内统一刊号:ISSN:31-1940/Q
  • 邮发代号:4-210
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,荷兰文摘与引文数据库,美国生物医学检索系统,美国剑桥科学文摘,美国科学引文索引(扩展库),美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),英国英国皇家化学学会文摘,中国北大核心期刊(2000版)
  • 被引量:5851