MicroRNAs (miRNAs ) 是小规章的分子 post-transcriptionallysuppressingmRNA 活动。在各种各样的有机体的许多 miRNAs 被克隆但是许多未知 miRNAs 遗体到我们描述从老鼠马头鱼尾的怪兽克隆六新 miRNAs 的 beidentified.Here。在他们之中,四没在老鼠米尔底被发现,但是与他们的人或老鼠相当或相同的事物相同,因此,他们被指定为 rno-miR-92b , rno-miR-146b , rno-let-7g ,和 rno-miR-551b.The 另外的二从 rno-miR-330 和 rno-miR-384 的已知的 miRNA 先锋的另外的手臂被导出,并且没在所有有机体的米尔底被发现。他们被指定为 rno-miR-330 * 并且这些 miRNAs 的 rno-miR-384 *.The 表示被跟踪 RNA 和教材扩展证实即时 reversetranscription 聚合酶链反应。这六 miRNAs 被表示在在马头鱼尾的怪兽显著地高级比在另外的纸巾包括服的外皮,心,肝,肺 andkidney.miR-384 * 是比在老鼠马头鱼尾的怪兽的 miR-384 更丰富的 10 次,但是小差别在 miR-330 之间被发现 * 并且在一样的织物的 miR-330 表示。
MicroRNAs (miRNAs) are small regulatory molecules post-transcriptionally suppressing mRNA activity. Many miRNAs in various organisms have been cloned but many unknown miRNAs remain to be identified. Here we describe the cloning of six new miRNAs from rat hippocampus. Among them, four were not found in the rat miRBase, but were identical to their human and/or mouse homolog, therefore they were designated as rno-miR-92b, rno-miR-146b, rno-let-7g, and rno-miR-55 lb. The other two were derived from the other arms of the known miRNA precursors of rno-miR-330 and rno-miR-384, and were not found in miRBase of all organisms. They were designated as rno-miR-330^* and rno-miR-384^*. The expression of these miRNAs was confirmed by RNA-tailing and primer-extension real-time reverse transcriptionpolymerase chain reaction. These six miRNAs were expressed at significantly higher levels in the hippocampus than in other tissues, including cerebral cortex, heart, liver, lung and kidney, miR-384^* was 10 times more abundant than miR-384 in rat hippocampus, but little difference was found between miR-330^* and miR-330 expression in the same tissue.