中文摘要：

目的 研究CpG寡聚脱氧核苷酸（CpG-ODN）对肺癌A549细胞化疗的影响,并探讨其可能的作用机制。方法 将肺癌A549细胞分为空白对照组、DDP组、CpG-ODN组、DDP＋CpG-ODN组、CpG-ODN＋DDP组。采用CCK-8比色法检测细胞的增殖情况,流式细胞仪检测细胞凋亡情况,RT-PCR检测TLR9 mRNA的表达,Western印迹法检测TLR9信号通路中My D88、AP-1的表达。结果 TLR9激活CpG ODN后,能显著提高细胞的增殖能力（P〈0.05）;CpG-ODN与CpG-ODN＋DDP两组细胞生长无明显差异（P〉0.05）;CpG-ODN＋DDP组细胞凋亡率较DDP组低（P〈0.05）,DDP＋CpG-ODN组细胞凋亡率较DDP组明显升高（P〈0.05）;DDP＋CpG-ODN组TLR9 mRNA较DDP组明显升高（P〈0.05）;DDP＋CpG组AP-1蛋白表达上调（P〈0.05）。结论 CpG-ODN激活TLR9信号通路后,可协同DDP促进细胞凋亡,提高顺铂化疗的敏感性。

英文摘要：

Objective To investigate the effect of TLR9 activator CpG-ODN on chemosensitivity of lung cancer A549 cells.Methods Cultured lung cancer A549 cells were divided into control group, DDP group, CpG-ODN group, DDP＋CpG-ODN group, CpG-ODN＋DDP group.Cell proliferation was analyzed with CCK-8 method;cell apoptosis was examined with flow cytometry;the mRNA expression of TLR9 was detected with RT-PCR;and the protein expression of MyD88 and AP-1 was detected with Western blotting.Results The cell proliferation was improved in CpG-ODN groups（ P 〈0.05）;there was no significant difference between CpG-ODN and CpG-ODN＋DDP groups（ P 〉0.05）.The apoptosis rate of CpG-ODN＋DDP was lower than that of DDP（ P 〈0.05）, and the apoptotic rate of DDP＋CpG-ODN was significantly higher than that of DDP（ P 〈0.05）.The expression of TLR9 mRNA in DDP＋CpG-ODN was higher than that in DDP group（ P 〈0.05）.The expression of AP-1 in DDP＋CpG group was up-regulated（ P 〈0.05）.Conclusion The data indicate that the CpG-ODN can increase cell apoptosis and enhance the sensitivity of lung cancer cells to cisplatin chemotherapy.