中文摘要：

模拟大脑内神经元和神经胶质细胞生长的分形环境，构筑具有分形特征的粗糙表面为神经胶质细胞生长提供一个仿生环境。本研究以C6神经胶质细胞为模型，分别在具有分形结构的烷基烯酮二聚体（AKD）表面，以及不具有分形结构的光滑AKD表面和传统的多聚赖氨酸（PLL）表面进行体外细胞培养，用相差显微镜和激光共聚焦显微镜分别观察细胞形状和细胞内微丝纤维排列，用ImageJ软件分析细胞的三维立体形状。结果显示，分形AKD表面诱导神经胶质细胞具有较好的三维立体形状，细胞内皮层肌动蛋白和应力纤维消失；光滑AKD表面与对照组PLL表面生长细胞的形状和细胞内微丝排列无明显差异。可见，粗糙的分形结构能够诱导c6细胞内微丝纤维重新排列，进而引起细胞具有较好的三维立体形状。

英文摘要：

Neurons and glial cells in brain are surrounded by a fractal environment, therefore, artificial fractal surface may provide such an biomimic environment for glial cell culture. In the present study, C6 glioma cells were cultured on the fractal alkyl ketene dimmer（AKD）, non-fractal AKD and Poty-L-Lysine （PLL）-coated surfaces. The cell morphologies were observed by phase-contrast microscopy. F-actin arrangements were analyzed by confoeal microscopy, three-dimensional cell shapes were constructed by Image J software. Experimental results showed that the fraetal surface of AKD induced C6 glioma cells to develop perfect three-dimensional shape, without cortical aetin and stress fibers in cytoplasm. However, no significant difference was obtained in the cells on the non-fractal and PLL- coated surface. In conclusion, the fractnral surface induced F-actin rearrangements of the cells to develop higher perfect three-dimensional shape.