中文摘要：

目的观察芪丹通脉片（QDTMT）对心肌梗死后气虚血瘀证大鼠缺血心肌血管新生的作用及对HIF-1α、VEGF表达的影响。方法以结扎SD大鼠左冠状动脉前降支，制备急性心肌梗死模型，随机分为假手术（Sham）组、梗死组（MI）组、MI＋芪丹通脉片小剂量（MI＋QDTMTL）组和MI＋芪丹通脉片大剂量（MI＋QDTMTH）组。术后24h各组均用生理盐水配制成等体积药液灌胃，4周后动态评价各组大鼠气虚血瘀表征变化，采用免疫组化染色方法检测梗死边缘区CD31的表达，测定各组大鼠缺血心肌中新生血管数、微血管密度，于第7日、14日及28日时检测心梗边缘区HIF-1α和VEGFmRNA的表达。结果（1）Sham组无气虚血瘀证相关表现，而MI组、MI＋QDTMTL组和MI＋QDTMTH组都有气虚血瘀的相关表现，且MI组表现最为明显。（2）MI＋QDTMTL组和MI＋QDTMTH组CD31的表达较MI组增强，且MI＋QDTMTH组高于MI＋QDTMTL组。（3）与MI组相比．MI＋QDTMTL组和MI＋QDTMTH组心梗边缘区新生血管数及微血管密度增加，且MI＋QDTMTH组高于MI＋QDTMTL组。（4）RT—PCR结果显示，心梗后7d、14d时MI＋QDTMTL组和MI＋QDTMTH组HIF-1α mRNA及VEGFmRNA相对表达量较MI组增高．28d时MI＋QDTMTL组和MI＋QDTMTH组HIF-1α mRNA及VEGFmRNA相对表达量较MI组降低。结论QDTMT能够改善心梗大鼠气虚血瘀的症状，增加心梗边缘区新生血管数和微血管密度，调控HIF-1α与VEGFmRNA的表达，为心梗后心功能的改善提供了新的治疗思路．其机制可能与活化HIF-1α信号调控HIF-1α mRNA及VEGFmRNA的表达有关。

英文摘要：

Objective： To study the effects of Qidantongmai Tablet on angiogenesis and expression of HIF-1α and VEGF in isehemic myoeardium of rats with myocardial infarction. Methods： Sprague-Dawley （SD） rats were subjected to MI by ligating the left anterior descending coronary and randomly divided into sham group,MI group ,low-dose QDTMT group （MI＋QDTMTL） and high-dose QDTMT （MI＋QDTMTH）. After 24 hours of ligation, equal volume of liquid in each group was administrated with normal saline for 4 weeks. Qixuxueyu characterization changes of rats in each group were dynamicly evaluated after 4 weeks. The expression of CD31 in area at risk was measured by immunohistochemical methods,and Microvascular vascular count （MVC）,Microvascular vascular density（MVD） in the ischemic myocardium were measured, at 7 d, 14 d and 28 d,the expression of HIF-1α mRNA and VEGFmRNA in the edge of infarc section were detected respectively. Results： （1）Qixuxueyu characterization changes： no Qixuxueyu disease-related performance of the sham group. But MI group,MI ＋ QDTMTL group and MI＋QDTMTH group has Qixuxueyu performance,and the performance of the MI group most obvious; （2）The expression of CD31 was higher in the MI＋QDTMTL groups and the MI＋QDTMTH group than the MI group,of which MI＋QDTMTL group is less than MI＋QDTMTH group. （3）Compared with the MI group,MVC and MVD in the edge of infarc section of the MI＋QDTMTL group and the MI＋QDTMTH group were significantly increased,of which the MI＋QDTMTH group is higher than MI＋QDTMTL group. （4）RT-PCR results showed that for the MI group, the expression of HIF-1α mRNA and VEGFmRNA of the MI＋QDTMTL group and MI＋QDTMTH group was increasing obviously at 7 d, 14 d after myocardial infarction, of which the MI＋QDTMTL group and MI＋ QDTMTH group was decreased at 28 d. Conclusion： Qidan Tongmai tablet can improve Qixuxueyu symptoms of MI rats,increase MVC and MVD in the edge of infarc section and regulate the expression of HIF-1α mRNA and