中文摘要：

利用RT–PCR和实时荧光定量PCR技术,对巴西橡胶树膜结合转录因子Hb NTL2进行克隆和表达,并通过酵母试验进行转录激活功能分析。结果表明,Hb NTL2基因的开放阅读框为1 380 bp,编码一个由459个氨基酸组成的蛋白,该蛋白属于NAC转录因子家族中的ANAC001亚族;在第6~172位氨基酸之间含有典型的NAC结构域,第433~453位氨基酸具有跨膜结构域,是膜结合NAC转录因子;Hb NTL2具有转录激活功能,转录激活区在C–末端;Hb NTL2在叶片的表达高于在橡胶树的其他部位,受割胶、茉莉酸（JA）和乙烯（ET）处理以及低温胁迫诱导。综合分析结果表明,Hb NTL2可能参与调控巴西橡胶树的生长发育和胁迫应答过程。

英文摘要：

The whole open reading frame （ORF） sequence of a membrane-associated NAC transcription factor gene, HbNTL2, was cloned and analyzed by using reverse transcription polymerase chain reaction （RT-PCR） and Real-time PCR, and yeast was adopted as a material in the transactivation assay. The results indicated that HbNTL2 belonged to the ANAC001 subfamily with an ORF of 1 380 bp and encoded a protein with 459 amino acids. A conserved NAC domain （12-167） and a transmembrane motif （433-453） were respectively identified at the N-terminal and C-terminal from motif analysis, the result of which indicated that HbNTL2 was a membrane-associated protein. Results from transactivation assay in yeast cells demonstrated that HbNTL2 assumed the function for transcription activator, and its activation domain was located at the highly divergent C-terminus. The expression level of HbNTL2 in leaves was higher than that in other parts of the rubber tree, and could be induced by tapping, jasmonic acid （JA）, ethyphon （ET） and cold stress. The results suggested that HbNTL2 might be play important roles in the regulation of growth, development and stress responses.