中文摘要：

淀粉酶产色链霉菌1628（Streptomyces diastatochromogenes）是一株具有广阔应用前景的生防菌株，其合成的主要活性物质为核苷类抗生素—丰加霉素。为了提高淀粉酶产色链霉菌的发酵水平，本研究采用单因素和正交实验设计，对菌株1628的发酵培养基和发酵条件进行了优化，优化后的最佳培养基配方为：可溶性淀粉1.5％、黄豆粉5％、FeSO4 0.1％、CaCO3 0.5％、NH4NO3 0.3％、KH2PO4 0.3％，最适初始pH7.0。在供试的发酵条件内，装液量60mL／250mL，发酵温度28℃，摇床转速180r／min，发酵时间为92h。活性物质丰加霉素的产量最高156.17mg·L^-1，比优化前提高了3.3倍。

英文摘要：

Streptomyces diastatochromogenes 1628 has broad applications in biocontrol field and mainy biosynthesized uncleosides antibiotics-toyocamycin. In order to improve the toyocamycin production, single- factor experiments and orthogonal tests were designed to optimize the fermentation medium and conditions for Streptornyces diastatochromogenes 1628. The optimum fermentation medium was soluble starch 1.5%,soybean meal 5%, FeSO4 0.1%, CaCO3 0.5%, NH4NO3 0.3%, and KH2PO4 0.3%. The optimum cultural conditions were initial pH7.0, capacity 60 mL/250 mL, temperature 28℃,180r·min^-1 for 92 h. Under optimized conditions, the output of toyocamycin is 3.3 times higher than that of the control.