从新疆某铀矿厂土样中分离出1株芽孢杆菌,初步确定为苏云金芽孢杆菌(Bt),命名为BRC-ZYR3.该菌株含有3种cry1类基因(cry1Ba、cry1Bb、cry1Be/1Bf)和2种cry9类基因(cry9Ca和cry9Da).Bt BRC-ZYR3可产生5种杀虫晶体蛋白,分别为130、70、50、30和25 ku.此外,该菌株能耐受25 mg·L-1Cr(VI),并在72 h内将其还原至3.8 mg·L-1.
A soil sample was collected from a factory of uranium-mine in Xinjiang Province, China. A new isolate, designated as BRC-ZYR3, was identified as Bacillus thuringiensis (Bt). The results showed that Bt BRC-ZYR3 harbored crylBa, crylBb, crylBe/1Bf, cry9Ca and cr)gDa genes and encoded 130, 70, 50, 30 and 25 ku insecticidal crystal proteins (ICPs). And Bt BRC- ZYR3 resistant to 25 mg· L-1 Cr(VI) could reduce Cr(VI) to 3.8 mg· L-1.