中文摘要：

目的研究家蝇抗菌肽基因attacin、cecropin、defensin在家蝇幼虫体内的空间转录情况。方法采用体外转录方法合成地高辛标记的cRNA探针,通过原位杂交方法检测微生物诱导前后家蝇幼虫体内attacin、cecropin、defensin变化情况。结果在未诱导的家蝇幼虫体内,体壁、中肠组织均没有检测到attacin、cecropin和defensin转录,在脂肪体中仅检测到cecropin和defensin的弱转录信号;在诱导后的家蝇幼虫中,体壁上检测到attacin和defensin强转录信号,cecropin没有转录,脂肪体中检测到attacin、cecropin和defensin强转录信号,而在中肠组织中检测到cecropin和defensin转录,没有attacin转录。结论经微生物诱导后,家蝇幼虫体内抗菌肽基因转录水平大大提高,为进一步研究家蝇抗菌肽在机体免疫中的作用及其机制打下了基础。

英文摘要：

The study aimed to study the spatial transcription of the housefly antimicrobial peptides,including attacin,cecropin,and defensin,in the housefly larvae.Digoxin-labeled cRNA probe was synthesized by in vitro transcription,and then used to detect attacin,cecropin,and defensin transcription in unchallenged and challenged housefly larva tissues by in situ hybridization.In the unchallenged larvae,attacin,cecropin,and defensin transcriptions were not found in the epithelia of the body wall and the midgut,while weak transcription of cecropin and defensin was detected in the fat body;in the challenged larvae,stronger transcription signals of attacin and defensin was detected in the epithelia of the body wall,but cecropin was not.Attacin,cecropin,and defensin transcriptions were found in the fat body;cecropin and defensin transcription was detected in the midgut,while attacin was not.The results mean that in the challenged housefly larvae,the transcription of antimicrobial peptides are increased greatly,which is a foundation to further study their role in the immune as well as the mechanism.