中文摘要：

目的：构建并鉴定含鼠bcl-2基因重组逆转录病毒。 方法：将抗凋亡基因bcl-2片段从载体pcDNA3．1中切下，定向插入逆转录病毒载体PLNCx2中，酶切鉴定；脂质体法将重组逆转录病毒转入包装细胞系PT67，G418筛选建立稳定表达bcl-2的细胞株。 结果：双酶切鉴定，成功构建重组逆转录病毒载体；重组逆转录病毒转入包装细胞PT67，经G418筛选。形成了抗性克隆，并测得病毒滴度为3×10^11cfu，L提示构建的含鼠bcl-2基因重组逆转录病毒载体及稳定的包装细胞系成功。 结论：含抗凋亡基因bcl-2重组逆转录病毒载体构建成功。

英文摘要：

AIM： To construct and confirm a recombinant retroviral vector of mice bc/-2 gene. METHODS： The bc/-2 gene segment was obtained by appropriate restriction enzymes, and then cloned into a retroviral vector PLNCX2 and retroviral vector plasmid DNA was analyzed after digested by appropriate restriction enzymes. The recombinant plasmid was then transferred into packaging cell PT67 with Lipofection method. Stable virus-producing cell line was obtained by screen of G418. RESULTS： The retroviral vector PLNCX2-bcl -2 was successfully constructed, and confirmed by enzyme digestion analysis. Anti-G418 positive clones were also achieved with a titer of 3× 10^11 cfu/L. CONCLUSION： Recombinant ratroviral vector of bcl-2 gene have been constructed successfully, and then it may be used in gene therapy for further of retinal denaturalization disorders.