中文摘要：

以苏云金芽孢杆菌Bt菌液为模版,用PCR扩增的方法克隆出两端带酶切位点XhoⅠ的Cry2Ab片段,与高效植物表达栽体pSR784d连接,并进行PCR和酶切鉴定。结果表明,克隆片段含有Cry2Ab基因、且连接方向正确,所构建植物表达载体Cry2Ab-pSR784d序列与相关元件正确。研究构建单独Cry2Ab植物表达载体,为转基因植物表达和通过转基因植物验证其功能奠定了基础。

英文摘要：

PCR was performed to amplify Cry2Ab gene from Bacillus thuringiensis. Then Cry2Ab gene was ligated to plant expression vector pSR784d with double restriction enzyme sites Xho I. The results showed that the Cry2Ab gene was cloned properly and the expression vector Cry2Ab-pSR784d was constructed successfully. This study constructed a plant expression vector for Cry2Ab transgenic plant and laid foundation for further related studies.